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231225s2019 xx |||||o 00| ||eng c |
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7 |
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|a 10.5713/ajas.18.0739
|2 doi
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|a pubmed25n0978.xml
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|a (DE-627)NLM293701857
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|a (NLM)30744366
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Lee, Joo Bin
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Effect of oocyte chromatin status in porcine follicles on the embryo development in vitro
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| 264 |
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1 |
|c 2019
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a OBJECTIVE: The main goal of this study was to provide a morphological indicator that could be used to select high-quality oocytes of appropriate meiotic and developmental capabilities in pig. The higher quality of immature oocytes, the higher success rates of in vitro maturation (IVM) and in vitro fertilization (IVF). Thus, prior to the IVM culture, it is important to characterize oocytes morphologically and biochemically in order to assess their quality. Two of the largest indicators of oocyte quality are the presence of cumulus cells and status of chromatin. To investigate the effects of porcine oocyte chromatin configurations on the developmental capacity of blastocysts, we assessed oocyte chromatin status according to follicle size and measured the developmental potency of blastocysts
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| 520 |
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|a METHODS: To sort by follicle size, we divided the oocytes into three groups (less than 1 mm, 1 to 3 mm, and more than 3 mm in diameter). To assess chromatin configuration, the oocytes were assessed for their stages (surrounded nucleolus [SN] germinal vesicle [GV], non-surrounded nucleolus [NSN] GV, GV breakdown, metaphase I [MI], pro-metaphase II [proMII], and metaphase II [MII]) at different maturation times (22, 44, and 66 h). To assess the development rate, oocytes of each follicle size were subjected to parthenogenetic activation for further development. Finally, GV oocytes were grouped by their chromatin configuration (SN, SN/NSN, and NSN) and their global transcriptional levels were measured
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|a RESULTS: SN GV oocytes were more suitable for IVF than NSN GV oocytes. Moreover, oocytes collected from the larger follicles had a greater distribution of SN GV oocytes and a higher developmental capacity during IVM, reaching MII more quickly and developing more often to blastocysts
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|a CONCLUSION: Porcine oocytes with high-level meiotic and developmental capacity were identified by analyzing the relationship between follicle size and chromatin configuration. The porcine oocytes from large follicles had a significantly higher SN status in which the transcription level was low and could be better in the degree of meiotic progression and developmental capacity
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| 650 |
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4 |
|a Journal Article
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4 |
|a Embryo Development
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| 650 |
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4 |
|a Follicle Size
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| 650 |
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4 |
|a Oocyte Maturation
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| 650 |
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4 |
|a Porcine Oocyte Chromatin
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| 700 |
1 |
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|a Lee, Min Gu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, Tao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shin, Hyeon Yeong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Jae Eun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kang, Jung Won
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jin, Dong-Il
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Asian-Australasian journal of animal sciences
|d 1998
|g 32(2019), 7 vom: 01. Juli, Seite 956-965
|w (DE-627)NLM098195883
|x 1011-2367
|7 nnns
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| 773 |
1 |
8 |
|g volume:32
|g year:2019
|g number:7
|g day:01
|g month:07
|g pages:956-965
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| 856 |
4 |
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|u http://dx.doi.org/10.5713/ajas.18.0739
|3 Volltext
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