|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM293694362 |
| 003 |
DE-627 |
| 005 |
20231225075855.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2010 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1094/PDIS-03-10-0183
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0978.xml
|
| 035 |
|
|
|a (DE-627)NLM293694362
|
| 035 |
|
|
|a (NLM)30743615
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Zheng, Lu
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Isolation, Purification, and Biological Activity of a Phytotoxin Produced by Stemphylium solani
|
| 264 |
|
1 |
|c 2010
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Revised 20.11.2019
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status PubMed-not-MEDLINE
|
| 520 |
|
|
|a Stemphylium solani, the causal agent of leaf blight of garlic (Allium sativum), produced phytotoxic metabolites in culture. A non-host-specific phytotoxin from culture filtrate of S. solani isolate DY-5, named SS-toxin, was extracted by ethyl acetate, isolated by bioassay-guided thin layer chromatography on silica gel, and purified by preparative liquid chromatography. SS-toxin produced necrotic lesions on detached garlic leaves, similar to that caused by S. solani. When wounded leaves were each treated with a 10-μl droplet of toxin, Changbanpo, a garlic cultivar susceptible to leaf blight, showed necrotic lesions at 11 μg/ml toxin, while a resistant cultivar, Qingganruanye, showed symptoms at 44 μg/ml. The effective doses causing 50% inhibition (EC50 values) of root growth and shoot elongation of the susceptible cultivar were 64.9 and 178.5 μg/ml, respectively. The SS-toxin significantly inhibited mitotic activity of root tip cells of the susceptible cultivar from 22 μg/ml and higher causing an abnormally high frequency of cells in interphase. Concentrations over 50 μg/ml of SS-toxin were found to be significantly toxic to total chlorophyll, both chlorophyll a and b, of the susceptible cultivar. The plasma membrane-cell wall interface, nuclear membranes, mitochondria, and chloroplasts were affected by SS-toxin in susceptible leaf cells. This is the first report of the purification and testing of a phytotoxin produced by S. solani
|
| 650 |
|
4 |
|a Journal Article
|
| 700 |
1 |
|
|a Lv, Rujing
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Huang, Junbin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Jiang, Daohong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hsiang, Tom
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 94(2010), 10 vom: 31. Okt., Seite 1231-1237
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
|
| 773 |
1 |
8 |
|g volume:94
|g year:2010
|g number:10
|g day:31
|g month:10
|g pages:1231-1237
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1094/PDIS-03-10-0183
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 94
|j 2010
|e 10
|b 31
|c 10
|h 1231-1237
|