Sensitive and Specific Detection of Xanthomonas oryzae pv. oryzae by Real-Time Bio-PCR Using Pathovar-Specific Primers Based on an rhs Family Gene

The present study describes bio-polymerase chain reaction (PCR) assays to detect bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae in rice. Successful control of X. oryzae. pv. oryzae requires a specific and reliable diagnostic tool. However, other X. oryzae pathovars are detected by cur...

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Bibliographische Detailangaben
Veröffentlicht in:	Plant disease. - 1997. - 95(2011), 5 vom: 14. Mai, Seite 589-594
1. Verfasser:	Cho, Min Seok (VerfasserIn)
Weitere Verfasser:	Kang, Man Jung, Kim, Chang Kug, Seol, Young-Joo, Hahn, Jang Ho, Park, Soo Chul, Hwang, Duk Ju, Ahn, Tae-Young, Park, Duck Hwan, Lim, Chun Keun, Park, Dong Suk
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2011
Zugriff auf das übergeordnete Werk:	Plant disease
Schlagworte:	Journal Article

Beschreibung
Zusammenfassung:	The present study describes bio-polymerase chain reaction (PCR) assays to detect bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae in rice. Successful control of X. oryzae. pv. oryzae requires a specific and reliable diagnostic tool. However, other X. oryzae pathovars are detected by currently available molecular and serological methods. In this study, SYBR Green real-time and conventional PCR primer sets were designed based on an rhs family gene of X. oryzae pv. oryzae KACC10331 because these genes are structurally diverse. The specificity of the primers was evaluated using purified DNA from 11 isolates of two X. oryzae pathovars, 21 other Xanthomonas species, and 4 other reference phytopathogenic bacteria and fungi. The assay was also able to detect at least two genome equivalents of cloned amplified target DNA using purified DNA. Thus, the SYBR Green real-time PCR-based method can be used for the rapid and specific detection of X. oryzae pv. oryzae and will potentially simplify and facilitate diagnosis and monitoring of this pathogen and guide plant disease management
Beschreibung:	Date Revised 20.11.2019 published: Print Citation Status PubMed-not-MEDLINE
ISSN:	0191-2917
DOI:	10.1094/PDIS-06-10-0399