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231225s2012 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-03-11-0263
|2 doi
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|a DE-627
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|a eng
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| 100 |
1 |
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|a Ochwo-Ssemakula, M
|e verfasserin
|4 aut
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| 245 |
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|a Characterization and Distribution of a Potyvirus Associated with Passion Fruit Woodiness Disease in Uganda
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| 264 |
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a This article describes the incidence and etiology of a viral disease of passion fruit in Uganda. Symptoms, including those characteristic of passion fruit woodiness disease (PWD), were observed on 32% of plants in producing areas. Electron microscopic observations of infected tissues revealed flexuous filaments of ca. 780 nm. Enzymelinked immunosorbent assays indicated a serological relationship with Cowpea aphid-borne mosaic virus (CABMV) and Passion fruit ringspot virus (PFRSV). In host range studies, only species in the families Solanaceae and Chenopodiaceae were susceptible, and neither Vigna unguiculata nor Phaseolus vulgaris became infected. Coat protein (CP) gene sequences of eight isolates exhibited features typical of potyviruses and were highly similar (88 to 100% identity). However, the sequences had limited sequence identity with CP genes of two of the three potyviruses reported to cause PWD: East Asian Passiflora virus and Passion fruit woodiness virus (PWV). Deduced amino acid sequences for the CP of isolates from Uganda had highest identity with Bean common mosaic necrosis virus (BCMNV) (72 to 79%, with evolutionary divergence values between 0.17 and 0.19) and CABMV (73 to 76%, with divergence values between 0.21 and 0.25). Based on these results and in accordance with International Committee for Taxonomy of Viruses criteria for species demarcation in the family Potyviridae, we conclude that a previously unreported virus causes viral diseases on passion fruit in Uganda. The name "Ugandan Passiflora virus" is proposed for this virus
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| 650 |
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|a Journal Article
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|a Sengooba, T
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hakiza, J J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Adipala, E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Edema, R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Redinbaugh, M G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Aritua, V
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Winter, S
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 96(2012), 5 vom: 01. Mai, Seite 659-665
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
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|g volume:96
|g year:2012
|g number:5
|g day:01
|g month:05
|g pages:659-665
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|u http://dx.doi.org/10.1094/PDIS-03-11-0263
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