Hyaloperonospora camelinae on Camelina sativa in Washington State : Detection, Seed Transmission, and Chemical Control

Camelina (Camelina sativa) plants with symptoms of downy mildew were obtained from three different locations in Washington State. Based on polymerase chain reaction (PCR) and sequencing of the internal transcribed spacer (ITS)1-5.8S-ITS2 region, the causal pathogen was identified as Hyaloperonospora...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Plant disease. - 1997. - 96(2012), 11 vom: 01. Nov., Seite 1670-1674
1. Verfasser: Babiker, E M (VerfasserIn)
Weitere Verfasser: Hulbert, S H, Paulitz, T C
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article
LEADER 01000naa a22002652 4500
001 NLM293535620
003 DE-627
005 20231225075528.0
007 cr uuu---uuuuu
008 231225s2012 xx |||||o 00| ||eng c
024 7 |a 10.1094/PDIS-02-12-0212-RE  |2 doi 
028 5 2 |a pubmed24n0978.xml 
035 |a (DE-627)NLM293535620 
035 |a (NLM)30727462 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Babiker, E M  |e verfasserin  |4 aut 
245 1 0 |a Hyaloperonospora camelinae on Camelina sativa in Washington State  |b Detection, Seed Transmission, and Chemical Control 
264 1 |c 2012 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Revised 20.11.2019 
500 |a published: Print 
500 |a Citation Status PubMed-not-MEDLINE 
520 |a Camelina (Camelina sativa) plants with symptoms of downy mildew were obtained from three different locations in Washington State. Based on polymerase chain reaction (PCR) and sequencing of the internal transcribed spacer (ITS)1-5.8S-ITS2 region, the causal pathogen was identified as Hyaloperonospora camelinae. The PCR primers consistently amplified 699-bp bands from the infected plants but not from the asymptomatic plants. A comparison of the sequences with those in GenBank revealed 100% sequence similarity to H. camelinae. Growth and development of the H. camelinae was observed in different tissues using light microscopy and scanning electron microscopy (SEM). Light microscopic observation revealed the presence of oospores in the infected leaves and SEM revealed the presence of conidia and conidiophores on the seed surface. To determine whether H. camelinae is a seed-transmitted pathogen, seed collected from infected plants were planted in Sunshine professional growing mix maintained in a growth chamber. Disease symptoms were observed in 96% of the seedlings compared with 3% of the seedlings grown from seed from asymptomatic plants, which indicates that H. camelinae is a seed-transmitted pathogen. Seed treated with mefenoxam, a fungicide specific for Oomycetes, significantly reduced the incidence of the disease 
650 4 |a Journal Article 
700 1 |a Hulbert, S H  |e verfasserin  |4 aut 
700 1 |a Paulitz, T C  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant disease  |d 1997  |g 96(2012), 11 vom: 01. Nov., Seite 1670-1674  |w (DE-627)NLM098181742  |x 0191-2917  |7 nnns 
773 1 8 |g volume:96  |g year:2012  |g number:11  |g day:01  |g month:11  |g pages:1670-1674 
856 4 0 |u http://dx.doi.org/10.1094/PDIS-02-12-0212-RE  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 96  |j 2012  |e 11  |b 01  |c 11  |h 1670-1674