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|a 10.1094/PDIS-03-12-0258-PDN
|2 doi
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|a eng
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| 100 |
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|a Ji, Y H
|e verfasserin
|4 aut
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|a First Report of Tomato yellow leaf curl virus Infecting Common Bean in China
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|c 2012
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| 336 |
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a Common bean (Phaseolus vulgaris) is one of the most economically important vegetable crops in China. In November 2011, symptoms with thickening and crumpling of leaves and stunting were observed on common bean with incidence rate of 50 to 70% in the fields of Huaibei, northern Anhui Province, China. Diseased common bean plants were found to be infested with large population of whiteflies (Bemisia tabaci), which induced leaf crumple symptoms in healthy common beans, suggesting begomovirus etiology. To identify possible begomoviruses, 43 symptomatic leaf samples from nine fields were collected and total DNA of each sample was extracted. PCR was performed using degenerate primers PA and PB to amplify a specific region covering AV2 gene of DNA-A and part of the adjacent intergenic region (2). DNA fragments were successfully amplified from 37 out of 43 samples and PCR amplicons of 31 samples were used for sequencing. Sequence alignments among them showed that the nucleotide sequence identity ranged from 99 to 100%, which implied that only one type of begomovirus might be present. Based on the consensus sequences, a primer pair MB1AbF (ATGTGGGATCCACTTCTAAATGAATTTCC) and MB1AsR (GCGTCGACAGTGCAAGACAAACTACTTGGGGACC) was designed and used to amplify the circular viral DNA genome. The complete genome (Accession No. JQ326957) was 2,781 nucleotides long and had the highest sequence identity (over 99%) with Tomato yellow leaf curl virus (TYLCV; Accession Nos. GQ352537 and GU199587). These samples were also examined by dot immunobinding assay using monoclonal antibody against TYLCV and results confirmed that TYLCV was present in the samples. These results demonstrated that the virus from common bean is an isolate of TYLCV, a different virus from Tomato yellow leaf curl China virus (TYLCCNV). TYLCV is a devastating pathogen causing significant yield losses on tomato in China since 2006 (4). The virus has also been reported from cowpea in China (1) and in common bean in Spain (3). To our knowledge, this is the first report of TYLCV infecting common bean in China. References: (1) F. M. Dai et al. Plant Dis. 95:362, 2011. (2) D. Deng et al. Ann. Appl. Biol. 125:327, 1994. (3) J. Navas-Castillo et al. Plant Dis. 83:29, 1999. (4) J. B. Wu et al. Plant Dis. 90:1359, 2006
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|a Journal Article
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|a Cai, Z D
|e verfasserin
|4 aut
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| 700 |
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|a Zhou, X W
|e verfasserin
|4 aut
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| 700 |
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|a Liu, Y M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xiong, R Y
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, T M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, W G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tao, X R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhou, Y J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 96(2012), 8 vom: 01. Aug., Seite 1229
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|g volume:96
|g year:2012
|g number:8
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|g pages:1229
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|u http://dx.doi.org/10.1094/PDIS-03-12-0258-PDN
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