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231225s2013 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-03-13-0232-PDN
|2 doi
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|a eng
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|a Svoboda, J
|e verfasserin
|4 aut
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|a First Report of Broad bean wilt virus-2 in Pepper in the Czech Republic
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|c 2013
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a In August 2012, field-grown pepper (Capsicum annuum) plants were inspected for the presence of viral diseases in Moravia, Czech Republic. Leaves taken from plants showing virus-like symptoms were examined for the presence of pepper viruses by double antibody sandwich (DAS)-ELISA. Of the 80 collected samples, 22 were infected with Cucumber mosaic virus, 17 with Potato virus Y, 13 with Broad bean wilt virus-1, and 11 with Broad bean wilt virus-2 (BBWV-2). The BBWV-2 positive samples were collected near the villages of Hrádek and Tasovice in Znojmo District and Loděnice in Brno-Country District. Six samples positive for BBWV-2 were also infected with one or two other viruses. BBWV-2 was mechanically transmitted from the field-collected samples where it was present alone onto Chenopodium quinoa plants. After systemic mottling emerged on indicator plants, symptomatic leaves were collected and the presence of BBWV-2 was confirmed by electron microscopy and reverse transcriptase (RT)-PCR. Isometric particles of about 25 nm corresponding in size and shape to the BBWV-2 virions (2) were observed. Total RNA was extracted from infected C. quinoa leaves, and RT-PCRs with primer pairs specific to four BBWV-2 genome regions (1) were carried out using oligo-dT and random hexamer mixtures for the cDNA synthesis. Amplicons of expected sizes, ~400 bp for Fab5'R1, ~460 bp for Pol2, ~500 bp for MP4, and ~250 bp for SCP were obtained for all samples. To the best of our knowledge, this is the first report of a natural occurrence of BBWV-2 in the Czech Republic. References: (1) R. M. Ferrer et al. Arch. Virol. 156:1445, 2011. (2) V. Lisa and G. Boccardo. The Plant Viruses 5:229. Plenum Press, New York, 1996
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|a Journal Article
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|a Leisova-Svobodova, L
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 97(2013), 9 vom: 05. Sept., Seite 1261
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|x 0191-2917
|7 nnns
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| 773 |
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|g volume:97
|g year:2013
|g number:9
|g day:05
|g month:09
|g pages:1261
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|u http://dx.doi.org/10.1094/PDIS-03-13-0232-PDN
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