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231225s2013 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-04-12-0344-RE
|2 doi
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|a pubmed24n0978.xml
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|a DE-627
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| 041 |
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|a eng
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| 100 |
1 |
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|a Xing, Junjie
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Analysis of Genetic and Molecular Identity Among Field Isolates of the Rice Blast Fungus with an International Differential System, Rep-PCR, and DNA Sequencing
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|c 2013
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
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|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a The Pi-ta gene deployed in southern U.S. rice germplasm is effective in preventing the infection by strains of Magnaporthe oryzae isolates that carry the avirulence (AVR) gene AVR-Pita1. In the present study, 169 isolates from rice (Oryza sativa) cultivars, with and without Pi-ta, were analyzed for their genetic identity using an international differential system, repetitive element-based polymerase chain reaction (Rep-PCR), and sequence analysis of PCR products of AVR-Pita1. These isolates belong to the races IA1, IB1, IB17, IC1, and IC17 of M. oryzae. These isolates were further classified into 15 distinct groups by Rep-PCR. There was a predominant group within each race. Pathogenicity assays on 'Katy' (Pi-ta) and 'M202' (pi-ta) rice determined that IC1 was virulent to Katy and M202; IB17, IC17, and most of IA1 and IB1 were avirulent to Katy and virulent to M202, suggesting that the Pi-ta gene in Katy is responsible for preventing infection by these isolates. Consistently, AVR-Pita1 was not amplified from 28 virulent isolates. One AVR-Pita1 allele was amplified by AVR-Pita1-specific primers in 78 avirulent isolates. Interestingly, different AVR-Pita1 alleles were found in each of the 12 avirulent isolates, as determined by DNA sequencing. Sequence analysis of 90 PCR products revealed 10 AVR-Pita1 haplotypes, 4 of which were new. In total, 12 amino acid changes were identified in the new variants when compared with the first described AVR-Pita sequence (AF207841). The finding of isolates with altered AVR-Pita1 from rice cultivars with and without Pi-ta suggests that these virulent isolates were adapted to the field environments in the southern United States. Further research will be needed to verify this prediction
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|a Journal Article
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| 700 |
1 |
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|a Jia, Yulin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Correll, James C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Fleet N
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cartwright, Richard
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cao, Mengliang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yuan, Longping
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 97(2013), 4 vom: 05. Apr., Seite 491-495
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
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|g volume:97
|g year:2013
|g number:4
|g day:05
|g month:04
|g pages:491-495
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| 856 |
4 |
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|u http://dx.doi.org/10.1094/PDIS-04-12-0344-RE
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