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|a 10.1094/PDIS-01-13-0048-RE
|2 doi
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|a eng
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| 100 |
1 |
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|a Liu, M-H
|e verfasserin
|4 aut
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|a Occurrence of Grapevine Leafroll-Associated Viruses in China
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| 264 |
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1 |
|c 2013
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|a Text
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|a ƒaComputermedien
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a To characterize the prevalence of viruses associated with grapevine leafroll disease in China, 249 grapevine (Vitis spp.) samples (86 popular cultivars and a rootstock) from 19 provinces and regions were collected and tested for Grapevine leafroll-associated virus 1 (GLRaV-1), GLRaV-2, GLRaV-3, GLRaV-4, and GLRaV-4 strain 5 by SYBR Green real-time reverse-transcription polymerase chain reaction (RT-PCR), and RT-PCR and sequencing. GLRaV-3 was found in 100% of the samples while GLRaV-1, GLRaV-2, and GLRaV-4 were detected in 24.9% (62/249), 15.3% (38/249), and 0.80% (2/249) of the samples, respectively. Single infections with GLRaV-3 were found in 66.3% (165/249) of the samples, and the remaining samples were mixed infections of GLRaV-3 with one or two other GLRaVs, those with GLRaV-1 being the most common (18.5%, 46/249). The genetic variability of Chinese GLRaV-3 isolates was characterized based on the coat protein (CP) gene. In total, 153 full-length CP gene sequences (94 sequences newly generated) of Chinese GLRaV-3 isolates from different grapevine-growing regions showed 89.3 to 100.0% and 92.7 to 100.0% identity at the nucleotide and amino acid levels, respectively. The average nucleotide diversity for the population of Chinese GLRaV-3 isolates was estimated at 0.037 (standard error = 0.0032). GLRaV-3 isolates from China segregated into five distinct phylogenetic groups and two novel recombination events were found in the viral population. This is the first and most extensive report of the prevalent species of GLRaV in China, which also provides an assessment of genetic variability of GLRaV-3 Chinese isolates
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| 650 |
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4 |
|a Journal Article
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| 700 |
1 |
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|a Li, M-J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Qi, H-H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, X-M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Q
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheng, Y-Q
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
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|g 97(2013), 10 vom: 05. Okt., Seite 1339-1345
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|g volume:97
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|g month:10
|g pages:1339-1345
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|u http://dx.doi.org/10.1094/PDIS-01-13-0048-RE
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