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231225s2014 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-07-13-0746-RE
|2 doi
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|a eng
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| 100 |
1 |
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|a Saito, Seiya
|e verfasserin
|4 aut
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| 245 |
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|a Selection, Fitness, and Control of Grape Isolates of Botrytis cinerea Variably Sensitive to Fenhexamid
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| 264 |
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|c 2014
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| 336 |
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|a Text
|b txt
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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| 500 |
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|a Date Revised 20.11.2019
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a Of 683 Botrytis cinerea isolates collected from a fungicide-trial vineyard, 31 were classified as putatively resistant to fenhexamid (50% effective concentration [EC50] ≥ 0.1 μg/ml). For the resistant isolates that survived and sporulated in culture, colony expansion and conidial germination frequency was significantly reduced relative to the mean of 30 representative baseline isolates (EC50 = 0.03 μg/ml). Grape berries were inoculated with four isolates representing a range of fenhexamid sensitivities and treated preventively or curatively with fenhexamid concentrations (150 to 600 mg/liter) representing 25 to 100% of the recommended rate. All treatments significantly delayed disease onset and progress caused by isolates with EC50 values of 0.03 and 0.15 μg/ml but provided little to no control of isolates with EC50 values of 0.32 and 62.5 μg/ml. The latter isolate exhibited a previously unreported F427V mutation of ERG27, an enzyme of ergosterol biosynthesis. In a duplex quantitative polymerase chain reaction test, the ratio of pathogen/host DNA increased significantly for 14 days after inoculation of untreated berries with a baseline isolate but declined slightly in berries treated with fenhexamid at 600 mg/liter 1 day post inoculation. In the vineyard, disease control was affected by the number and rate of fenhexamid applications but B. cinerea isolates with EC50 ≥ 0.1 μg/ml were not preferentially selected
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| 650 |
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|a Journal Article
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1 |
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|a Cadle-Davidson, Lance
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wilcox, Wayne F
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 98(2014), 2 vom: 01. Feb., Seite 233-240
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|x 0191-2917
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|g volume:98
|g year:2014
|g number:2
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|g pages:233-240
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|u http://dx.doi.org/10.1094/PDIS-07-13-0746-RE
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