Quick and Accurate Detection and Quantification of Magnaporthe oryzae in Rice Using Real-Time Quantitative Polymerase Chain Reaction

Quick and Accurate Detection and Quantification of Magnaporthe oryzae in Rice Using Real-Time Quantitative Polymerase Chain Reaction

Rice blast, caused by Magnaporthe oryzae, is one of the most severe fungal diseases in rice worldwide. In this study, we developed methods to quickly and accurately detect and quantify M. oryzae in the pure cultures of the fungus, rice plants, and rice seed by using SYBR Green I of the real-time qua...

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Veröffentlicht in:Plant disease. - 1997. - 99(2015), 2 vom: 31. Feb., Seite 219-224
1. Verfasser: Sun, Geng (VerfasserIn)
Weitere Verfasser: Liu, Jinliang, Li, Guihua, Zhang, Xianghui, Chen, Tingting, Chen, Jingyuan, Zhang, Hao, Wang, Dongping, Sun, Fengjie, Pan, Hongyu
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article
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520 |a Rice blast, caused by Magnaporthe oryzae, is one of the most severe fungal diseases in rice worldwide. In this study, we developed methods to quickly and accurately detect and quantify M. oryzae in the pure cultures of the fungus, rice plants, and rice seed by using SYBR Green I of the real-time quantitative polymerase chain reaction (qPCR). Results of absolute qPCR show that Magnaporthe oryzae can be detected at as low as 6.9 × 10-5 ng of genomic DNA. Results also show that all 10 varieties of rice seed examined in this study contain this fungus, indicating that M. oryzae is generally widespread in rice seed. We report the quantification of DNA of M. oryzae in rice leaves collected in the field, instead of in the lab, using relative qPCR by using rice actin gene as a housekeeping gene. Our results show great practical significance because we would know the potential fungal infection even before planting 
650 4 |a Journal Article 
700 1 |a Liu, Jinliang  |e verfasserin  |4 aut 
700 1 |a Li, Guihua  |e verfasserin  |4 aut 
700 1 |a Zhang, Xianghui  |e verfasserin  |4 aut 
700 1 |a Chen, Tingting  |e verfasserin  |4 aut 
700 1 |a Chen, Jingyuan  |e verfasserin  |4 aut 
700 1 |a Zhang, Hao  |e verfasserin  |4 aut 
700 1 |a Wang, Dongping  |e verfasserin  |4 aut 
700 1 |a Sun, Fengjie  |e verfasserin  |4 aut 
700 1 |a Pan, Hongyu  |e verfasserin  |4 aut 
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