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01000naa a22002652 4500 |
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NLM293059926 |
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DE-627 |
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20231225074503.0 |
| 007 |
cr uuu---uuuuu |
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231225s2017 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1094/PDIS-07-16-0951-RE
|2 doi
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| 028 |
5 |
2 |
|a pubmed24n0976.xml
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| 035 |
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|a (DE-627)NLM293059926
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| 035 |
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|a (NLM)30678568
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Guo, Mengpei
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Biological and Molecular Characteristics of a Novel Partitivirus Infecting the Edible Fungus Lentinula edodes
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| 264 |
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1 |
|c 2017
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Revised 20.11.2019
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| 500 |
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|a published: Print-Electronic
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| 500 |
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a A new partitivirus named Lentinula edodes partitivirus 1 (LePV1) was isolated from a diseased L. edodes strain with severe degeneration of the mycelium and imperfect browning in bag cultures. The nucleotide sequences of LePV1 dsRNA-1 and dsRNA-2 were determined; they were 2,382 bp and 2,245 bp in length, and each contained a single ORF encoding RNA-dependent RNA polymerase (RdRp) and coat protein (CP), respectively. The purified virus preparation contained isometric particles 34 nm in diameter encapsidating these dsRNAs. Phylogenetic analyses showed LePV1 to be a new member of Betapartitivirus, with the RdRp sequence most closely related to Grapevine partitivirus. RT-PCR analysis showed that 27 of the 56 Chinese L. edodes core collection strains carry LePV1, with the virus being more common in wild strains than cultivated strains. In addition, qPCR analysis suggested that coinfection with L. edodes mycovirus HKB (LeV-HKB) could increase replication of the RdRp gene of LePV1. This study may be essential for the development of more accurate disease diagnostics and the formulation of control strategies for viral diseases in L. edodes
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| 650 |
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4 |
|a Journal Article
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| 700 |
1 |
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|a Bian, Yinbing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Jinjie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Gangzheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ma, Xiaolong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Zhangyi
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 101(2017), 5 vom: 01. Mai, Seite 726-733
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
8 |
|g volume:101
|g year:2017
|g number:5
|g day:01
|g month:05
|g pages:726-733
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1094/PDIS-07-16-0951-RE
|3 Volltext
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 101
|j 2017
|e 5
|b 01
|c 05
|h 726-733
|