Links Between Genetic Groups, Host Specificity, and Ergot-Alkaloid Profiles within Claviceps purpurea (Fr.) Tul. on Slovenian Grasses

In the present study, the genetic relationships and ergot-alkaloid production of the fungus Claviceps purpurea on grasses were investigated, to determine any associations between grass host specificity, ergot-alkaloid production, and geographic origin. C. purpurea sclerotia were obtained from wild a...

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Veröffentlicht in:Plant disease. - 1997. - 102(2018), 7 vom: 24. Juli, Seite 1334-1340
1. Verfasser: Likar, Matevž (VerfasserIn)
Weitere Verfasser: Grandič, Marjana, Strajn, Breda Jakovac, Kos, Katarina, Celar, Franci Aco
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA, Fungal DNA, Ribosomal Spacer Ergolines Ergot Alkaloids Ergotamines ergocristine 3E58HO2T0U ergocryptine mehr... 511-09-1 ergosine 6WF908487H ergocornine 7W36B25464 Ergotamine PR834Q503T Ergonovine WH41D8433D
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520 |a In the present study, the genetic relationships and ergot-alkaloid production of the fungus Claviceps purpurea on grasses were investigated, to determine any associations between grass host specificity, ergot-alkaloid production, and geographic origin. C. purpurea sclerotia were obtained from wild and cultivated grasses along a 300-km climatic gradient, from sub-Mediterranean to continental climates. Twenty-one infected grass samples provided 39 sclerotia for analysis of the ergot alkaloids ergometrine, ergosine, ergotamine, ergocornine, ergocryptine, and ergocristine, and their "-inine" epimers, using liquid chromatography-tandem mass spectrometry. C. purpurea ribosomal DNA underwent molecular classification to determine any grass host or geographic specificity of ergot-alkaloid composition for the different operational taxonomic units. Molecular analysis of sclerotia ribosomal DNA showed three genetic groups, with some associations with specific grass host taxonomic groups. The ergot-alkaloid composition data were in agreement with the data obtained by molecular methods. The most frequent ergot-alkaloid epimers were ergocristine, and ergosine. The total ergot-alkaloid concentrations in sclerotia varied from 59 to 4,200 mg kg-1, which corresponds to 0.059 to 4.2 mg kg-1 in animal feed (assuming ergot alkaloids at 1,000 mg kg-1 sclerotia). Therefore, grasses can be associated with significant levels of ergot alkaloids. In addition, the ergot-alkaloid compositions of C. purpurea sclerotia can be different for infections with different C. purpurea genetic groups, because these show different ergot-alkaloid compositions 
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650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Ergot Alkaloids  |2 NLM 
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650 7 |a Ergotamine  |2 NLM 
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700 1 |a Grandič, Marjana  |e verfasserin  |4 aut 
700 1 |a Strajn, Breda Jakovac  |e verfasserin  |4 aut 
700 1 |a Kos, Katarina  |e verfasserin  |4 aut 
700 1 |a Celar, Franci Aco  |e verfasserin  |4 aut 
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