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DE-627 |
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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-12-17-1988-RE
|2 doi
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|a pubmed24n0976.xml
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|a (NLM)30673557
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Al Rwahnih, Maher
|e verfasserin
|4 aut
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| 245 |
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|a Discovery of Viruses and Virus-Like Pathogens in Pistachio using High-Throughput Sequencing
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| 264 |
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|c 2018
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 28.02.2019
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|a Date Revised 28.02.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Pistachio (Pistacia vera L.) trees from the National Clonal Germplasm Repository (NCGR) and orchards in California were surveyed for viruses and virus-like agents by high-throughput sequencing (HTS). Analyses of sequence information from 60 trees identified a novel virus, provisionally named "Pistachio ampelovirus A" (PAVA), in the NCGR that showed low amino acid sequence identity (approximately 42%) compared with members of the genus Ampelovirus (family Closteroviridae). A putative viroid, provisionally named "Citrus bark cracking viroid-pistachio" (CBCVd-pis), was also found in the NCGR and showed approximately 87% similarity to Citrus bark cracking viroid (CBCVd, genus Cocadviroid, family Pospiviroidae). Both PAVA and CBCVd-pis were graft transmissible to healthy UCB-1 hybrid rootstock seedlings (P. atlantica × P. integerrima). A field survey of 123 trees from commercial orchards found no incidence of PAVA but five (4%) samples were infected with CBCVd-pis. Of 675 NCGR trees, 16 (2.3%) were positive for PAVA and 172 (25.4%) were positive for CBCVd-pis by reverse-transcription polymerase chain reaction. Additionally, several contigs across multiple samples exhibited significant sequence similarity to a number of other plant virus species in different families. These findings require further study and confirmation. This study establishes the occurrence of viral and viroid populations infecting pistachio trees
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Capsid Proteins
|2 NLM
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| 700 |
1 |
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|a Rowhani, Adib
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Westrick, Nathaniel
|e verfasserin
|4 aut
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| 700 |
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|a Stevens, Kristian
|e verfasserin
|4 aut
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| 700 |
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|a Diaz-Lara, Alfredo
|e verfasserin
|4 aut
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| 700 |
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|a Trouillas, Florent P
|e verfasserin
|4 aut
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| 700 |
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|a Preece, John
|e verfasserin
|4 aut
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| 700 |
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|a Kallsen, Craig
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Farrar, Kristen
|e verfasserin
|4 aut
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| 700 |
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|a Golino, Deborah
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 102(2018), 7 vom: 24. Juli, Seite 1419-1425
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
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|g volume:102
|g year:2018
|g number:7
|g day:24
|g month:07
|g pages:1419-1425
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|u http://dx.doi.org/10.1094/PDIS-12-17-1988-RE
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