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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b03266
|2 doi
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|a pubmed25n0976.xml
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|a (NLM)30673280
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|a DE-627
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|a eng
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|a Wasilewska, Monika
|e verfasserin
|4 aut
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|a Human Serum Albumin Adsorption Kinetics on Silica
|b Influence of Protein Solution Stability
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 22.06.2020
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|a Date Revised 22.06.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Adsorption kinetics of human serum albumin (HSA) on silica substrates was studied using optical waveguide lightmode spectroscopy (OWLS) and quartz microbalance (QCM) techniques. Measurements were performed at pH 3.5, 5.6, and 7.4 for various bulk suspension concentrations and ionic strengths. The diffusion coefficient measurements showed that for pH 3.5 the HSA molecules are stable for NaCl concentrations from 10-3 to 0.15 M. This allowed us to precisely determine the mass transfer rate coefficients for the OWLS and QCM cells. The experimental data were adequately interpreted in terms of a hybrid random sequential adsorption model. The OWLS maximum coverage of HSA at pH 3.5, which is equal to 1.3 mg m-2, agrees with the QCM result and with previous results derived from streaming potential measurements. Thus, the results obtained at pH 3.5 served as reference data for the analysis of adsorption kinetics at higher pHs. In this way, it was confirmed that the adsorption kinetics of HSA molecules at pH 5.6 and 7.4 was considerably slower than at pH 3.5. This effect was attributed to aggregation of HSA solutions and interpreted in terms of a theoretical model combining the Smoluchowski aggregation theory with the convective diffusion mass transfer theory. New analytical equations were derived that can be used for the interpretation of other protein adsorption from unstable solutions
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Sodium Chloride
|2 NLM
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|a 451W47IQ8X
|2 NLM
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|a Silicon Dioxide
|2 NLM
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|a 7631-86-9
|2 NLM
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|a Serum Albumin, Human
|2 NLM
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|a ZIF514RVZR
|2 NLM
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|a Adamczyk, Zbigniew
|e verfasserin
|4 aut
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|a Pomorska, Agata
|e verfasserin
|4 aut
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|a Nattich-Rak, Małgorzata
|e verfasserin
|4 aut
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| 700 |
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|a Sadowska, Marta
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 35(2019), 7 vom: 19. Feb., Seite 2639-2648
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:35
|g year:2019
|g number:7
|g day:19
|g month:02
|g pages:2639-2648
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|u http://dx.doi.org/10.1021/acs.langmuir.8b03266
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