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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1111/nph.15690
|2 doi
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|a pubmed25n0976.xml
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|a (DE-627)NLM292919522
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|a (NLM)30664249
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Kosmacz, Monika
|e verfasserin
|4 aut
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| 245 |
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|a Protein and metabolite composition of Arabidopsis stress granules
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.02.2020
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
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|a Stress granules (SGs) are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Despite their importance for stress adaptation, no complete proteome composition has been reported for plant SGs. In this study, we addressed the existing gap. Importantly, we also provide evidence for metabolite sequestration within the SGs. To isolate SGs we used Arabidopsis seedlings expressing green fluorescent protein (GFP) fusion of the SGs marker protein, Rbp47b, and an experimental protocol combining differential centrifugation with affinity purification (AP). SGs isolates were analysed using mass spectrometry-based proteomics and metabolomics. A quarter of the identified proteins constituted known or predicted SG components. Intriguingly, the remaining proteins were enriched in key enzymes and regulators, such as cyclin-dependent kinase A (CDKA), that mediate plant responses to stress. In addition to proteins, nucleotides, amino acids and phospholipids also accumulated in SGs. Taken together, our results indicated the presence of a preexisting SG protein interaction network; an evolutionary conservation of the proteins involved in SG assembly and dynamics; an important role for SGs in moderation of stress responses by selective storage of proteins and metabolites
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis thaliana
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|a CDKA1
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|a affinity purification
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|a stress granule extraction
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|a stress granule metabolome
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|a stress granule proteome
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|a stress response
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a Proteome
|2 NLM
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| 700 |
1 |
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|a Gorka, Michał
|e verfasserin
|4 aut
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| 700 |
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|a Schmidt, Stephan
|e verfasserin
|4 aut
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| 700 |
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|a Luzarowski, Marcin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Moreno, Juan C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Szlachetko, Jagoda
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Leniak, Ewa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sokolowska, Ewelina M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sofroni, Kostika
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schnittger, Arp
|e verfasserin
|4 aut
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| 700 |
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|a Skirycz, Aleksandra
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1984
|g 222(2019), 3 vom: 15. Mai, Seite 1420-1433
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:222
|g year:2019
|g number:3
|g day:15
|g month:05
|g pages:1420-1433
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|u http://dx.doi.org/10.1111/nph.15690
|3 Volltext
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|d 222
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