Interaction between Brassica yellows virus silencing suppressor P0 and plant SKP1 facilitates stability of P0 in vivo against degradation by proteasome and autophagy pathways

Interaction between Brassica yellows virus silencing suppressor P0 and plant SKP1 facilitates stability of P0 in vivo against degradation by proteasome and autophagy pathways

© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1984. - 222(2019), 3 vom: 15. Mai, Seite 1458-1473
1. Verfasser: Li, Yuanyuan (VerfasserIn)
Weitere Verfasser: Sun, Qian, Zhao, Tianyu, Xiang, Haiying, Zhang, Xiaoyan, Wu, Zhanyu, Zhou, Cuiji, Zhang, Xin, Wang, Ying, Zhang, Yongliang, Wang, Xianbing, Li, Dawei, Yu, Jialin, Dinesh-Kumar, Savithramma P, Han, Chenggui
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Nicotiana benthamiana F-box-like motif P0 SKP1 polerovirus protein stability suppression of RNA silencing Plant Proteins mehr... S-Phase Kinase-Associated Proteins Viral Proteins Proteasome Endopeptidase Complex EC 3.4.25.1
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520 |a P0 protein of some polerovirus members can target ARGONAUTE1 (AGO1) to suppress RNA silencing. Although P0 harbors an F-box-like motif reported to be essential for interaction with S phase kinase-associated protein 1 (SKP1) and RNA silencing suppression, it is the autophagy pathway that was shown to contribute to AGO1 degradation. Therefore, the role of P0-SKP1 interaction in silencing suppression remains unclear. We conducted global mutagenesis and comparative functional analysis of P0 encoded by Brassica yellows virus (BrYV) (P0Br ). We found that several residues within P0Br are required for local and systemic silencing suppression activities. Remarkably, the F-box-like motif mutant of P0Br , which failed to interact with SKP1, is destabilized in vivo. Both the 26S proteasome system and autophagy pathway play a role in destabilization of the mutant protein. Furthermore, silencing of a Nicotiana benthamiana SKP1 ortholog leads to the destabilization of P0Br . Genetic analyses indicated that the P0Br -SKP1 interaction is not directly required for silencing suppression activity of P0Br , but it facilitates stability of P0Br to ensure efficient RNA silencing suppression. Consistent with these findings, efficient systemic infection of BrYV requires P0Br . Our results reveal a novel strategy used by BrYV for facilitating viral suppressors of RNA silencing stability against degradation by plant cells 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Nicotiana benthamiana 
650 4 |a F-box-like motif 
650 4 |a P0 
650 4 |a SKP1 
650 4 |a polerovirus 
650 4 |a protein stability 
650 4 |a suppression of RNA silencing 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a S-Phase Kinase-Associated Proteins  |2 NLM 
650 7 |a Viral Proteins  |2 NLM 
650 7 |a Proteasome Endopeptidase Complex  |2 NLM 
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700 1 |a Sun, Qian  |e verfasserin  |4 aut 
700 1 |a Zhao, Tianyu  |e verfasserin  |4 aut 
700 1 |a Xiang, Haiying  |e verfasserin  |4 aut 
700 1 |a Zhang, Xiaoyan  |e verfasserin  |4 aut 
700 1 |a Wu, Zhanyu  |e verfasserin  |4 aut 
700 1 |a Zhou, Cuiji  |e verfasserin  |4 aut 
700 1 |a Zhang, Xin  |e verfasserin  |4 aut 
700 1 |a Wang, Ying  |e verfasserin  |4 aut 
700 1 |a Zhang, Yongliang  |e verfasserin  |4 aut 
700 1 |a Wang, Xianbing  |e verfasserin  |4 aut 
700 1 |a Li, Dawei  |e verfasserin  |4 aut 
700 1 |a Yu, Jialin  |e verfasserin  |4 aut 
700 1 |a Dinesh-Kumar, Savithramma P  |e verfasserin  |4 aut 
700 1 |a Han, Chenggui  |e verfasserin  |4 aut 
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