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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b03718
|2 doi
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|a pubmed24n0975.xml
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|a DE-627
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|a eng
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| 100 |
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|a Baxter, Alexis J
|e verfasserin
|4 aut
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|a Modulation of Cu2+ Binding to Sphingosine-1-Phosphate by Lipid Charge
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Revised 20.11.2019
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Sphingosine-1-phosphate (S1P) is a sphingolipid metabolite that is thought to participate in the regulation of many physiological processes and may play a key role in several diseases. Herein, we found that Cu2+ binds tightly to supported lipid bilayers (SLBs) containing S1P. Specifically, we demonstrated via fluorescence assays that Cu2+-S1P binding was bivalent and sensitive to the concentration of S1P in the SLB. In fact, the apparent equilibrium dissociation constant, KDApp, tightened by a factor of 132 from 4.5 μM to 34 nM as the S1P density was increased from 5.0 to 20 mol %. A major driving force for this apparent tightening was the more negative surface potential with increasing S1P concentration. This potential remained unaltered upon Cu2+ binding at pH 7.4 because two protons were released for every Cu2+ that bound. At pH 5.4, however, Cu2+ could not outcompete protons for the amine and no binding occurred. Moreover, at pH 9.4, the amine was partially deprotonated before Cu2+ binding and the surface potential became more positive on binding. The results for Cu2+-S1P binding were reminiscent of those for Cu2+-phosphatidylserine binding, where a carboxylate group helped to deprotonate the amine. In the case of S1P, however, the phosphate needed to bear two negative charges to facilitate amine deprotonation in the presence of Cu2+
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Santiago-Ruiz, Adriana N
|e verfasserin
|4 aut
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| 700 |
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|a Yang, Tinglu
|e verfasserin
|4 aut
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| 700 |
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|a Cremer, Paul S
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 35(2019), 3 vom: 22. Jan., Seite 824-830
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:35
|g year:2019
|g number:3
|g day:22
|g month:01
|g pages:824-830
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|u http://dx.doi.org/10.1021/acs.langmuir.8b03718
|3 Volltext
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