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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2018.12.028
|2 doi
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|a pubmed24n0975.xml
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|a (DE-627)NLM292623321
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|a (NLM)30634134
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|a (PII)S0981-9428(18)30575-8
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Kou, Meng
|e verfasserin
|4 aut
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|a A novel glutathione S-transferase gene from sweetpotato, IbGSTF4, is involved in anthocyanin sequestration
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 22.01.2019
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2019 Elsevier Masson SAS. All rights reserved.
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|a Anthocyanins are synthesized by multi-enzyme complexes localized at the cytoplasmic surface of the endoplasmic reticulum (synthesis site), and transported to the destination site, the vacuole. Three mechanisms for the vacuolar accumulation of anthocyanin in plant species have been proposed. Previous studies have indicated that glutathione S-transferase (GST) genes from model and ornamental plants are involved in anthocyanin transportation. In the present study, an anthocyanin-related GST, IbGSTF4, was identified and characterized based on transcriptome results. Phylogenetic analysis revealed that IbGSTF4 was most closely correlated to PhAN9 and CkmGST3, the anthocyanin-related GST of Petunia hybrida and Cyclamen. Furthermore, the expression analysis revealed that IbGSTF4 is strongly expressed in pigmented tissues, when compared to green organs, which is in agreement to the ability to correlate with anthocyanin accumulation. A GST activity assay uncovered that the IbGST4 protein owned similar activities with the GST family. Furthermore, the molecular functional complementation of Arabidopsis thaliana mutant tt19 demonstrated that IbGSTF4 might play a vital role in the vacuole sequestration of anthocyanin in sweetpotato. Moreover, the dual luciferase assay revealed that the LUC driven by the promoter of IbGSTF4 could not be directly activated by IbMYB1, suggesting that the regulatory mechanism of anthocyanin accumulation and sequestration in sweetpotato was intricate
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|a Journal Article
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|a Anthocyanin transportation
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|a Glutathione S-transferase
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|a Sweetpotato
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|a Anthocyanins
|2 NLM
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|a DNA, Plant
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Glutathione Transferase
|2 NLM
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| 650 |
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|a EC 2.5.1.18
|2 NLM
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| 700 |
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|a Liu, Ya-Ju
|e verfasserin
|4 aut
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|a Li, Zong-Yun
|e verfasserin
|4 aut
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|a Zhang, Yun-Gang
|e verfasserin
|4 aut
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|a Tang, Wei
|e verfasserin
|4 aut
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|a Yan, Hui
|e verfasserin
|4 aut
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|a Wang, Xin
|e verfasserin
|4 aut
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|a Chen, Xiao-Guang
|e verfasserin
|4 aut
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|a Su, Zai-Xing
|e verfasserin
|4 aut
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|a Arisha, Mohamed Hamed
|e verfasserin
|4 aut
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|a Li, Qiang
|e verfasserin
|4 aut
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| 700 |
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|a Ma, Dai-Fu
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 135(2019) vom: 15. Feb., Seite 395-403
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:135
|g year:2019
|g day:15
|g month:02
|g pages:395-403
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|u http://dx.doi.org/10.1016/j.plaphy.2018.12.028
|3 Volltext
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|d 135
|j 2019
|b 15
|c 02
|h 395-403
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