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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b03789
|2 doi
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|a pubmed24n0973.xml
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|a (DE-627)NLM292148356
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|a (NLM)30585494
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Sekiyama, Shota
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Delayed Increase in Near-Infrared Fluorescence in Cultured Murine Cancer Cells Labeled with Oxygen-Doped Single-Walled Carbon Nanotubes
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.11.2019
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|a Date Revised 27.11.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The labeling technique for cells with over-thousand-nanometer near-infrared (OTN-NIR) fluorescent probes has attracted much attention for in vivo deep imaging for cell tracking and cancer metastasis, because of low scattering and absorption of OTN-NIR light by biological tissues. However, the intracellular behavior following the uptake of the single-walled carbon nanotubes (SWCNTs), an OTN-NIR fluorophore, remains unknown. The aim of this study is to investigate the time-dependent change in OTN-NIR fluorescence images of cultured murine cancer cells (Colon-26) following treatment with a recently developed OTN-NIR fluorescent probe, epoxide-type oxygen-doped SWCNTs (o-SWCNTs). The o-SWCNTs were synthesized by oxygenation of SWCNTs by ozone under ultraviolet irradiation and were dispersed in an aqueous solution of N-(carbonyl-methoxypolyethyleneglycol 2000)-1,2-distearoyl- sn-glycero-3-phosphoethanolamine to prepare biocompatible o-SWCNTs (o-SWCNT-PEG). OTN-NIR fluorescent o-SWCNT-PEG showed an abnormal behavior following cellular uptake. OTN-NIR fluorescence was not observed in the cells after 24 h incubation with the o-SWCNT-PEG, but clearly increased with longer incubation time from three days after the treatment. This result was further confirmed by Raman microscopy, suggesting that OTN-NIR fluorescence intensity was associated with the cellular uptake of the o-SWCNT-PEG. These results suggest that the Colon-26 cells were successfully labeled by the o-SWCNT-PEG that emit OTN-NIR fluorescence. The o-SWCNT-PEG may aggregate in the cells over time, which could favor their internalization. This delayed concentration followed by a long retention of the o-SWCNT-PEG in cells will facilitate further biotechnological applications of the o-SWCNTs to in vivo deep OTN-NIR fluorescent imaging
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fluorescent Dyes
|2 NLM
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|a Nanotubes, Carbon
|2 NLM
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|a Polyethylene Glycols
|2 NLM
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|a 3WJQ0SDW1A
|2 NLM
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|a Ozone
|2 NLM
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|a 66H7ZZK23N
|2 NLM
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|a Oxygen
|2 NLM
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|a S88TT14065
|2 NLM
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| 700 |
1 |
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|a Umezawa, Masakazu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Iizumi, Yoko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ube, Takuji
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Okazaki, Toshiya
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kamimura, Masao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Soga, Kohei
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 35(2019), 3 vom: 22. Jan., Seite 831-837
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:35
|g year:2019
|g number:3
|g day:22
|g month:01
|g pages:831-837
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|u http://dx.doi.org/10.1021/acs.langmuir.8b03789
|3 Volltext
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|d 35
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|h 831-837
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