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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2018.11.004
|2 doi
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|a pubmed24n0972.xml
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|a (NLM)30537612
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|a (PII)S0176-1617(18)30414-0
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Ćosić, Tatjana
|e verfasserin
|4 aut
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|a Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 22.01.2019
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2018 Elsevier GmbH. All rights reserved.
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|a Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species
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|a Journal Article
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|a Cytokinin
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|a Gene expression
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|a Kohlrabi
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|a de novo shoot organogenesis
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|a Raspor, Martin
|e verfasserin
|4 aut
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| 700 |
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|a Savić, Jelena
|e verfasserin
|4 aut
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| 700 |
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|a Cingel, Aleksandar
|e verfasserin
|4 aut
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| 700 |
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|a Matekalo, Dragana
|e verfasserin
|4 aut
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| 700 |
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|a Zdravković-Korać, Snežana
|e verfasserin
|4 aut
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| 700 |
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|a Ninković, Slavica
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 232(2019) vom: 20. Jan., Seite 257-269
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:232
|g year:2019
|g day:20
|g month:01
|g pages:257-269
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|u http://dx.doi.org/10.1016/j.jplph.2018.11.004
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