A polyphasic approach to the delimitation of diatom species : a case study for the genus Pinnularia (Bacillariophyta)

© 2018 Phycological Society of America.

Bibliographische Detailangaben
Veröffentlicht in:Journal of phycology. - 1966. - 55(2019), 2 vom: 15. Apr., Seite 365-379
1. Verfasser: Kollár, Jan (VerfasserIn)
Weitere Verfasser: Pinseel, Eveline, Vanormelingen, Pieter, Poulíčková, Aloisie, Souffreau, Caroline, Dvořák, Petr, Vyverman, Wim
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Journal of phycology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't LSU rDNA Pinnularia subgibba SSU rDNA cox1 diatoms molecular taxonomy psbA rbcL mehr... species diversity DNA, Ribosomal
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520 |a Diatoms are one of the most abundant and arguably the most species-rich group of protists. Diatom species delimitation has often been based exclusively on the recognition of morphological discontinuities without investigation of other lines of evidence. Even though DNA sequences and reproductive experiments have revealed several examples of (pseudo)cryptic diversity, our understanding of diatom species boundaries and diversity remains limited. The cosmopolitan pennate raphid diatom genus Pinnularia represents one of the most taxon-rich diatom genera. In this study, we focused on the delimitation of species in one of the major clades of the genus, the Pinnularia subgibba group, based on 105 strains from a worldwide origin. We compared genetic distances between the sequences of seven molecular markers and selected the most variable pair, the mitochondrial cox1 and nuclear encoded LSU rDNA, to formulate a primary hypothesis on the species limits using three single-locus automated species delimitation methods. We compared the DNA-based primary hypotheses with morphology and with other available lines of evidence. The results indicate that our data set comprised 15 species of the P. subgibba group. The vast majority of these taxa have an uncertain taxonomic identity, suggesting that several may be unknown to science and/or members of (pseudo)cryptic species complexes within the P. subgibba group 
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650 4 |a diatoms 
650 4 |a molecular taxonomy 
650 4 |a psbA 
650 4 |a rbcL 
650 4 |a species diversity 
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700 1 |a Pinseel, Eveline  |e verfasserin  |4 aut 
700 1 |a Vanormelingen, Pieter  |e verfasserin  |4 aut 
700 1 |a Poulíčková, Aloisie  |e verfasserin  |4 aut 
700 1 |a Souffreau, Caroline  |e verfasserin  |4 aut 
700 1 |a Dvořák, Petr  |e verfasserin  |4 aut 
700 1 |a Vyverman, Wim  |e verfasserin  |4 aut 
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