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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1111/nph.15635
|2 doi
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|a pubmed24n0972.xml
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|a (DE-627)NLM291667317
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|a (NLM)30536576
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a He, Qin
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Phytophthora infestans effector SFI3 targets potato UBK to suppress early immune transcriptional responses
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.02.2020
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.
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|a The potato blight agent Phytophthora infestans secretes a range of RXLR effectors to promote disease. Recent evidence indicates that some effectors suppress early pattern-triggered immunity (PTI) following perception of microbe-associated molecular patterns (MAMPs). Phytophthora infestans effector PiSFI3/Pi06087/PexRD16 has been previously shown to suppress MAMP-triggered pFRK1-Luciferase reporter gene activity. How PiSFI3 suppresses immunity is unknown. We employed yeast-two-hybrid (Y2H) assays, co-immunoprecipitation, transcriptional silencing by RNA interference and virus-induced gene silencing (VIGS), and X-ray crystallography for structure-guided mutagenesis, to investigate the function of PiSFI3 in targeting a plant U-box-kinase protein (StUBK) to suppress immunity. We discovered that PiSFI3 is active in the host nucleus and interacts in yeast and in planta with StUBK. UBK is a positive regulator of specific PTI pathways in both potato and Nicotiana benthamiana. Importantly, it contributes to early transcriptional responses that are suppressed by PiSFI3. PiSFI3 forms an unusual trans-homodimer. Mutation to disrupt dimerization prevents nucleolar localisation of PiSFI3 and attenuates both its interaction with StUBK and its ability to enhance P. infestans leaf colonisation. PiSFI3 is a 'WY-domain' RXLR effector that forms a novel trans-homodimer which is required for its ability to suppress PTI via interaction with the U-box-kinase protein StUBK
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a disease resistance
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|a effector-triggered susceptibility
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|a pathogenicity
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|a potato late blight
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|a virulence
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|a Proteins
|2 NLM
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|a Flagellin
|2 NLM
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|a 12777-81-0
|2 NLM
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| 650 |
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|a Green Fluorescent Proteins
|2 NLM
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|a 147336-22-9
|2 NLM
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| 650 |
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|a Protein Kinases
|2 NLM
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| 650 |
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|a EC 2.7.-
|2 NLM
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| 700 |
1 |
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|a McLellan, Hazel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hughes, Richard K
|e verfasserin
|4 aut
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1 |
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|a Boevink, Petra C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Armstrong, Miles
|e verfasserin
|4 aut
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| 700 |
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|a Lu, Yuan
|e verfasserin
|4 aut
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1 |
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|a Banfield, Mark J
|e verfasserin
|4 aut
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1 |
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|a Tian, Zhendong
|e verfasserin
|4 aut
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| 700 |
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|a Birch, Paul R J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 222(2019), 1 vom: 01. Apr., Seite 438-454
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:222
|g year:2019
|g number:1
|g day:01
|g month:04
|g pages:438-454
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/nph.15635
|3 Volltext
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|d 222
|j 2019
|e 1
|b 01
|c 04
|h 438-454
|