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231225s2018 xx |||||o 00| ||chi c |
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|a 10.3760/cma.j.issn.0578-1310.2018.12.010
|2 doi
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|a pubmed24n0971.xml
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|a (NLM)30518009
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Luo, Y
|e verfasserin
|4 aut
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| 245 |
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|a Effect of miR-21 on the expression of interleukin-10 in B cell of patients with Henoch-Schonlein purpura
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.04.2019
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|a Date Revised 04.12.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a Objective: To investigate the effect of microRNAs (miR)-21 on the expression of interleukin (IL)-10 in B cell of patients with Henoch-Schonlein purpura (HSP). Methods: From March 2016 to January 2017, twenty-four children with HSP hospitalized in rheumatology and immunology department of Shenzhen Children's Hospital were enrolled into the study, including 12 males and 12 females. Patients were divided into purpura nephritis group (HSPN, 14 cases) and non-nephritis group (NHSPN, 10 cases). The age-matched 34 healthy children were included as the control group for prospective cohort study. The expression levels of IL-10 in peripheral blood B cells (CD19(+)), transitional B cells (CD19(+) CD24(hi)CD38(hi)) and naïve B cells (CD19(+)CD24(int)CD38(int)) from patients with HSP and healthy children were detected by flow cytometry (FCM). Expression of microRNAs related to IL-10 in B cells were quantitated by real-time PCR, including miR-21-5p, miR-106a-5p, miR-98-3p, miR-142-3p, miR-142-5p, miR-98-5p, miR-155-5p and miR-let7b-5p. Agomir negative control-FAM and agomir-21-5p-FAM were transfected into B cells from patients with HSP. The uptake of miRNA by B cells was observed by laser scanning confocal microscope and FCM, and the expression of IL-10 was detected by FCM after transfection. For quantitative data of normal distribution, t test was used for two samples comparison and multiple comparisons among three groups were conducted by ANOVA. Spearman test was used for correlation analysis. Results: (1) The CD19(+) B cells and its two populations at different differentiation stages all could express IL-10. The expression levels of IL-10 in three B cell populations in patients were significantly lower than those in healthy controls (1.4±0.2 vs. 2.4±0.3, t=3.501, P<0.01; 1.2±0.2 vs. 2.2±0.3, t=2.688, P<0.05; 1.6±0.3 vs. 2.7±0.4, t=2.498, P<0.05). Compared with healthy control and NHSPN groups, the expression of IL-10 in CD19(+) B cells from patients within HSPN group was the lowest, and the difference was statistically significant (1.1±0.2 vs. 2.4±0.3, 1.8±0.3, t=4.006, 2.362, P<0.001, P<0.05). (2) The expression of miR-21-5p in B cell in patients with HSPN was lower than that in healthy control group (1.2±0.9 vs. 3.5±2.8, t=2.962, P<0.01). There was no significant change in the other microRNAs. (3) The expression of IL-10 was positively correlated with the expression of miR-21-5p in the B cells of patients with HSP (r=0.778, P<0.001). (4) The expression of IL-10 in B cells of miR-21-5p group was significantly higher than that in negative control group (2.7±0.2 vs. 1.6±0.3, t=3.091, P<0.05). Conclusion: The insufficient expression of miR-21-5p in peripheral blood B cells of patients with HSP is one of the reasons for the reduction of IL-10 expression in B cells
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|a Journal Article
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|a B-lymphocytes
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4 |
|a Interleukin-10
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4 |
|a MicroRNAs
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| 650 |
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|a Purpura, schoenlein-henoch
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| 650 |
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7 |
|a MIRN21 microRNA, human
|2 NLM
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| 650 |
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|a MicroRNAs
|2 NLM
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| 650 |
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|a Interleukin-10
|2 NLM
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| 650 |
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|a 130068-27-8
|2 NLM
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| 700 |
1 |
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|a Huang, Y Y
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jin, Y
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, C R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, J
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Zhonghua er ke za zhi = Chinese journal of pediatrics
|d 1960
|g 56(2018), 12 vom: 02. Dez., Seite 939-944
|w (DE-627)NLM136249191
|x 0578-1310
|7 nnns
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| 773 |
1 |
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|g volume:56
|g year:2018
|g number:12
|g day:02
|g month:12
|g pages:939-944
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| 856 |
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|u http://dx.doi.org/10.3760/cma.j.issn.0578-1310.2018.12.010
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