Identification and characterization of the rice pre-harvest sprouting mutants involved in molybdenum cofactor biosynthesis

Identification and characterization of the rice pre-harvest sprouting mutants involved in molybdenum cofactor biosynthesis

© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 222(2019), 1 vom: 24. Apr., Seite 275-285
1. Verfasser: Liu, Xin (VerfasserIn)
Weitere Verfasser: Wang, Jing, Yu, Yang, Kong, Lina, Liu, Yimei, Liu, Zhiqi, Li, Hongyu, Wei, Piwei, Liu, Minglong, Zhou, Hai, Bu, Qingyun, Fang, Jun
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Oryza sativa MoCo-dependent enzyme abiotic stress tolerance cofactor for nitrate reductase and xanthine dehydrogenase (CNX) molybdenum cofactor (MoCo) pre-harvest sprouting (PHS) Coenzymes Metalloproteins mehr... Molybdenum Cofactors Plant Proteins Pteridines molybdenum cofactor ATN6EG42UQ
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520 |a © 2018 The Authors. New Phytologist © 2018 New Phytologist Trust. 
520 |a In cereal crops, ABA deficiency during seed maturation phase causes pre-harvest sprouting (PHS), and molybdenum cofactor (MoCo) is required for ABA biosynthesis. Here, two rice PHS mutants F254 and F5-1 were characterized. In addition to the PHS, these mutants showed pleiotropic phenotypes such as twisting and slender leaves, and then died when the seedling developed to four or five leaves. Map-based cloning showed that OsCNX6 and OsCNX1 encoding homologs of MoaE and MoeA were responsible for F254 and F5-1 mutants, respectively. Genetic complementation indicated that OsCNX6 not only rescued the PHS and seedling lethal phenotype of the cnx6 mutant, but also recovered the MoCo-dependent enzyme activities such as xanthine dehydrogenase (XDH), aldehyde oxidase (AO), nitrate reductase (NR) and sulfite oxidase (SO). Expression pattern showed that OsCNX6 was richly expressed in seed during embryo maturation by quantitative reverse transcriptase PCR and RNA in situ hybridization. Furthermore, the OsCNX6 overexpression plants can significantly enhance the MoCo-dependent enzyme activities, and improved the osmotic and salt stress tolerance without unfavorable phenotypes. Collectively, these data indicated that OsCNX6 participated in MoCo biosynthesis, and is essential for rice development, especially for seed dormancy and germination, and OsCNX6 could be an effective target for improving abiotic stress tolerance in rice 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Oryza sativa 
650 4 |a MoCo-dependent enzyme 
650 4 |a abiotic stress tolerance 
650 4 |a cofactor for nitrate reductase and xanthine dehydrogenase (CNX) 
650 4 |a molybdenum cofactor (MoCo) 
650 4 |a pre-harvest sprouting (PHS) 
650 7 |a Coenzymes  |2 NLM 
650 7 |a Metalloproteins  |2 NLM 
650 7 |a Molybdenum Cofactors  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Pteridines  |2 NLM 
650 7 |a molybdenum cofactor  |2 NLM 
650 7 |a ATN6EG42UQ  |2 NLM 
700 1 |a Wang, Jing  |e verfasserin  |4 aut 
700 1 |a Yu, Yang  |e verfasserin  |4 aut 
700 1 |a Kong, Lina  |e verfasserin  |4 aut 
700 1 |a Liu, Yimei  |e verfasserin  |4 aut 
700 1 |a Liu, Zhiqi  |e verfasserin  |4 aut 
700 1 |a Li, Hongyu  |e verfasserin  |4 aut 
700 1 |a Wei, Piwei  |e verfasserin  |4 aut 
700 1 |a Liu, Minglong  |e verfasserin  |4 aut 
700 1 |a Zhou, Hai  |e verfasserin  |4 aut 
700 1 |a Bu, Qingyun  |e verfasserin  |4 aut 
700 1 |a Fang, Jun  |e verfasserin  |4 aut 
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773 1 8 |g volume:222  |g year:2019  |g number:1  |g day:24  |g month:04  |g pages:275-285 
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