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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2018.07.021
|2 doi
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|a pubmed25n0969.xml
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|a (DE-627)NLM290980585
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|a (NLM)30466600
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|a (PII)S0168-9452(18)30627-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Yu, Zhenming
|e verfasserin
|4 aut
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|a The GDP-mannose transporter gene (DoGMT) from Dendrobium officinale is critical for mannan biosynthesis in plant growth and development
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.12.2018
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2018 Elsevier B.V. All rights reserved.
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|a Dendrobium officinale is a precious traditional Chinese medicinal herb because it is abundant in mannose-containing polysaccharides (MCPs). GDP-mannose transporter (GMT), which translocates GDP-mannose into the Golgi lumen, is indispensable for the biosynthesis of MCPs. In this study, we found that the dominant polysaccharides in D. officinale were MCPs in a range of varieties and different physiological phases. After a positive correlation between the accumulation of mannose and the transcript levels of candidate GMT genes was found, three GMT genes (DoGMT1-3) were identified in D. officinale. DoGMT1, DoGMT2 and DoGMT3 exhibited the highest transcript level in stem that an organ for MCPs storage. All three DoGMT proteins were targeted to Golgi apparatus, and had a GDP binding domain (GXL/VNK) that was homologous to a specially characterized GMT protein GONST1 in Arabidopsis thaliana. Moreover, DoGMT1, DoGMT2 and DoGMT3 complemented a GDP-mannose transport-defective yeast mutant (vrg4-2), meanwhile they also demonstrated a higher GDP-mannose uptake activity. Therefore, we conclude that DoGMT1, DoGMT2 and DoGMT3 are able to transport GDP-mannose while the expression patterns of these genes correspond to the accumulation of MCPs in D. officinale. These findings support the importance of GMT genes from D. officinale in the biosynthesis of MCPs
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|a Journal Article
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|a Dendrobium officinale
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|a GDP-mannose transporter
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|a Mannose
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|a Water-soluble polysaccharide
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| 650 |
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|a Carrier Proteins
|2 NLM
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|a GDP-mannose transporter
|2 NLM
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| 650 |
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|a Mannans
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Polysaccharides
|2 NLM
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| 650 |
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|a Mannose
|2 NLM
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| 650 |
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|a PHA4727WTP
|2 NLM
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| 700 |
1 |
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|a He, Chunmei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Teixeira da Silva, Jaime A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Luo, Jianping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Ziyin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Duan, Jun
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 277(2018) vom: 27. Dez., Seite 43-54
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
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|g volume:277
|g year:2018
|g day:27
|g month:12
|g pages:43-54
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|u http://dx.doi.org/10.1016/j.plantsci.2018.07.021
|3 Volltext
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