Cassette hybridization for vector assembly application in antibody chain shuffling

Gene assembly methods are an integral part of molecular cloning experiments. The majority of existing vector assembly methods stipulate a need for exonucleases, endonucleases and/or the use of single-stranded DNA as starting materials. Here, we introduced a vector assembly method that employs conven...

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Veröffentlicht in:	BioTechniques. - 1993. - 65(2018), 5 vom: 16. Nov., Seite 269-274
1. Verfasser:	Lai, Jing Yi (VerfasserIn)
Weitere Verfasser:	Loh, Qiuting, Choong, Yee Siew, Lim, Theam Soon
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2018
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't DNA assembly PCR-based cloning antibody chain shuffling homologous assembly scFv Antibodies, Monoclonal Peptide Library Single-Chain Antibodies mehr... DNA 9007-49-2