|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM290046742 |
| 003 |
DE-627 |
| 005 |
20250224073111.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2019 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/jfd.12902
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n0966.xml
|
| 035 |
|
|
|a (DE-627)NLM290046742
|
| 035 |
|
|
|a (NLM)30370695
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Ma, Jie
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Assessment of cross-protection to heterologous strains of Flavobacterium psychrophilum following vaccination with a live-attenuated coldwater disease immersion vaccine
|
| 264 |
|
1 |
|c 2019
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 14.01.2019
|
| 500 |
|
|
|a Date Revised 30.09.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2018 John Wiley & Sons Ltd.
|
| 520 |
|
|
|a Bacterial coldwater disease, caused by Flavobacterium psychrophilum, remains one of the most significant bacterial diseases of salmonids worldwide. A previously developed and reported live-attenuated immersion vaccine (F. psychrophilum; B.17-ILM) has been shown to confer significant protection to salmonids. To further characterize this vaccine, a series of experiments were carried out to determine the cross-protective efficacy of this B.17-ILM vaccine against 9 F. psychrophilum isolates (representing seven sequence types/three clonal complexes as determined by multilocus sequence typing) in comparison with a wild-type virulent strain, CSF-259-93. To assess protection, 28-day experimental challenges of rainbow trout (Oncorhynchus mykiss) fry were conducted following immersion vaccinations with the B.17-ILM vaccine. F. psychrophilum strains used in challenge trials were isolated from several fish species across the globe; however, all were found to be virulent in rainbow trout. The B.17-ILM vaccine provided significant protection against all strains, with relative percent survival values ranging from 51% to 72%. All vaccinated fish developed an adaptive immune response (as measured by F. psychrophilum-specific antibodies) that increased out to the time of challenge (8 weeks postimmunization). Previous studies have confirmed that antibody plays an important role in protection against F. psychrophilum challenge; therefore, specific antibodies to the B.17-ILM vaccine strain appear to contribute to the cross-protection observed to heterologous strain. The ability of such antibodies to bind to similar antigenic regions for all strains was confirmed by western blot analyses. Results presented here support the practical application of this live-attenuated vaccine, and suggest that it will be efficacious even in aquaculture operations affected by diverse strains of F. psychrophilum
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Flavobacterium psychrophilum
|
| 650 |
|
4 |
|a attenuated vaccine
|
| 650 |
|
4 |
|a cross-protection
|
| 650 |
|
4 |
|a virulent strains
|
| 650 |
|
7 |
|a Antibodies, Bacterial
|2 NLM
|
| 650 |
|
7 |
|a Bacterial Vaccines
|2 NLM
|
| 650 |
|
7 |
|a Vaccines, Attenuated
|2 NLM
|
| 700 |
1 |
|
|a Bruce, Timothy J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Sudheesh, Ponnerassery S
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Knupp, Christopher
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Loch, Thomas P
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Faisal, Mohamed
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Cain, Kenneth D
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 42(2019), 1 vom: 15. Jan., Seite 75-84
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
|
| 773 |
1 |
8 |
|g volume:42
|g year:2019
|g number:1
|g day:15
|g month:01
|g pages:75-84
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/jfd.12902
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 42
|j 2019
|e 1
|b 15
|c 01
|h 75-84
|