Application of next-generation sequencing technologies to conservation of wood-inhabiting fungi

© 2018 Society for Conservation Biology.

Bibliographische Detailangaben
Veröffentlicht in:Conservation biology : the journal of the Society for Conservation Biology. - 1999. - 33(2019), 3 vom: 01. Juni, Seite 716-724
1. Verfasser: Purahong, Witoon (VerfasserIn)
Weitere Verfasser: Wubet, Tesfaye, Krüger, Dirk, Buscot, François
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Conservation biology : the journal of the Society for Conservation Biology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't ITS conservación de microbios conservation strategy deadwood deadwood enrichment enriquecimiento de la madera muerta estrategia de conservación forest management mehr... madera muerta manejo de bosques microbial conservation next-generation sequencing secuenciación de nueva generación 二代测序 保护策略 微生物保护 枯木 枯木富集 森林管理 转录间隔区 (ITS)
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520 |a Next-generation sequencing (NGS) has significantly increased knowledge of microbial communities and their distribution. However, it is still not common to apply NGS technology to microbial conservation. We sought to use NGS technologies to evaluate conservation strategies for wood-inhabiting fungi. Evaluating a deadwood experiment 3 years after it was established, we specifically examined which tree species combinations promoted the highest richness of wood-inhabiting fungi. Deadwood enrichment was an effective strategy and logs of 6 tree species, either those with the highest wood-inhabiting fungal α and γ diversity or those with the highest β diversity, maintained >1,000 operational taxonomic units (OTUs) spread over a wide range of taxonomic groups. In comparison, a conservation strategy based only on the results of sporocarp surveys yielded 591 OTUs. This result highlights the need to use NGS approaches to inform microbial conservation strategies. We also determined that 5 tree species with the highest saproxylic beetle γ diversity simultaneously conserved wood-inhabiting fungi. Apart from deadwood volume, we suggest data on deadwood quality and species also be included as indicators, especially for wood-inhabiting fungal diversity, and incorporated quickly in forest assessment and monitoring systems in Central Europe 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a ITS 
650 4 |a conservación de microbios 
650 4 |a conservation strategy 
650 4 |a deadwood 
650 4 |a deadwood enrichment 
650 4 |a enriquecimiento de la madera muerta 
650 4 |a estrategia de conservación 
650 4 |a forest management 
650 4 |a madera muerta 
650 4 |a manejo de bosques 
650 4 |a microbial conservation 
650 4 |a next-generation sequencing 
650 4 |a secuenciación de nueva generación 
650 4 |a 二代测序 
650 4 |a 保护策略 
650 4 |a 微生物保护 
650 4 |a 枯木 
650 4 |a 枯木富集 
650 4 |a 森林管理 
650 4 |a 转录间隔区 (ITS) 
700 1 |a Wubet, Tesfaye  |e verfasserin  |4 aut 
700 1 |a Krüger, Dirk  |e verfasserin  |4 aut 
700 1 |a Buscot, François  |e verfasserin  |4 aut 
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773 1 8 |g volume:33  |g year:2019  |g number:3  |g day:01  |g month:06  |g pages:716-724 
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