Waste office paper : A potential feedstock for cellulase production by a novel strain Bacillus velezensis ASN1
Copyright © 2018 Elsevier Ltd. All rights reserved.
| Veröffentlicht in: | Waste management (New York, N.Y.). - 1999. - 79(2018) vom: 01. Sept., Seite 491-500 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | Waste management (New York, N.Y.) |
| Schlagworte: | Journal Article Bacillus velezensis Bioconversion Cellulase Optimization Waste office paper EC 3.2.1.4 Nitrogen N762921K75 |
| Zusammenfassung: | Copyright © 2018 Elsevier Ltd. All rights reserved. This paper reports the cellulase (FPase) production by newly isolated Bacillus velezensis ASN1 using waste office paper (WOP) as feedstock and optimization of production conditions through two level factorial design, steepest ascent/descent and second order response surface methodology (RSM). Various fermentation parameters, like chemical factors (potassium dihydrogen phosphate, potassium chloride, yeast extract, magnesium sulphate, sodium nitrate, Tween 80, and waste office paper), physical factors (temperature, pH and time) and biological factor (inoculum size) were examined using two level full factorial design to check the key factors significantly affecting the cellulase production. The central composite design (CCD) was used to optimize the vital fermentation parameters, such as carbon (WOP), nitrogen, pH, and inoculum concentration in the medium for achieving higher cellulase production. The optimum medium composition was found to be WOP (9 g/L), sodium nitrate (0.35 g/L), inoculum size (6.56%) and pH 4.72. The model prediction of 2.46 U/mL cellulase activity at optimum conditions was verified experimentally as 2.42 U/mL |
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| Beschreibung: | Date Completed 12.09.2019 Date Revised 12.09.2019 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1879-2456 |
| DOI: | 10.1016/j.wasman.2018.08.014 |