Effect of arsenate on endogenous levels of cytokinins with different existing forms in two Pteris species

Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 132(2018) vom: 05. Nov., Seite 652-659
1. Verfasser: Li, Qinchun (VerfasserIn)
Weitere Verfasser: Wang, Hongbin, Wang, Haijuan, Li, Yang, Wang, Zhongzhen, Zhang, Xuemei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Arsenic Conjugates Free forms Hyperaccumulator Phytohormone Arsenates Cytokinins Pigments, Biological
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520 |a Copyright © 2018 Elsevier Masson SAS. All rights reserved. 
520 |a Our previous results showed that content of trans-zeatin (tZ) increases in leaves of heavy metal hyperaccumulators but decreases in non-hyperaccumulators growing in multiple heavy metal polluted soils. However, the relationship between arsenic (As) accumulation and endogenous forms of cytokinins (CTKs) in As hyperaccumulators remains unknown. Here a hydroponic experiment was conducted to compare the CTK forms in the As hyperaccumulator Pteris cretica var. nervosa and non-hyperaccumulator Pteris ensiformis under arsenate stress (0, 2, 5, and 10 mg L-1). A simple and cost-effective procedure for the determination of CTK forms in plants was established, and a stepwise regression analysis was used to study the relationship among total As contents and different forms of endogenous CTKs in fronds of two plants. The results showed that the optimized chromatographic parameters were Zobax SB-C18 column (5 μm × 4.6 mm × 250 mm), UV detection detector at 269 nm, a flow rate of 0.6 mL min-1, constant temperature of 45 °C and gradient elution with methanol-acetonitrile-1% acetic acid. Contents of chlorophylls in the fronds of P. ensiformis were significantly decreased with addition of As compared to P. cretica var. nervosa. Furthermore, the total As content in fronds of P. cretica var. nervosa was positively correlated to the contents of N6-(2-isopentenyl) adenine-7-β-D-glucoside (iP7G) and N6-(2-isopentenyl) adenosine (iPR). However, the total As content in fronds of P. ensiformis was negatively correlated to its trans-zeatin riboside (ZR) content. Therefore, iP7G and iPR could positively improve As accumulation by P. cretica var. nervosa 
650 4 |a Journal Article 
650 4 |a Arsenic 
650 4 |a Conjugates 
650 4 |a Free forms 
650 4 |a Hyperaccumulator 
650 4 |a Phytohormone 
650 7 |a Arsenates  |2 NLM 
650 7 |a Cytokinins  |2 NLM 
650 7 |a Pigments, Biological  |2 NLM 
700 1 |a Wang, Hongbin  |e verfasserin  |4 aut 
700 1 |a Wang, Haijuan  |e verfasserin  |4 aut 
700 1 |a Li, Yang  |e verfasserin  |4 aut 
700 1 |a Wang, Zhongzhen  |e verfasserin  |4 aut 
700 1 |a Zhang, Xuemei  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 132(2018) vom: 05. Nov., Seite 652-659  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
773 1 8 |g volume:132  |g year:2018  |g day:05  |g month:11  |g pages:652-659 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2018.10.009  |3 Volltext 
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