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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2018.09.019
|2 doi
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|a pubmed24n1224.xml
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|a (DE-627)NLM289478707
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|a (NLM)30312968
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|a (PII)S0176-1617(18)30657-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Saad, Rania Ben
|e verfasserin
|4 aut
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|a A stress-associated protein, LmSAP, from the halophyte Lobularia maritima provides tolerance to heavy metals in tobacco through increased ROS scavenging and metal detoxification processes
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.01.2019
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2018 Elsevier GmbH. All rights reserved.
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|a Agricultural soil pollution by heavy metals is a severe global ecological problem. We recently showed that overexpression of LmSAP, a member of the stress-associated protein (SAP) gene family isolated from Lobularia maritima, in transgenic tobacco led to enhanced tolerance to abiotic stress. In this study, we characterised the response of LmSAP transgenic tobacco plants to metal stresses (cadmium (Cd), copper (Cu), manganese (Mn), and zinc (Zn)). In L. maritima, LmSAP expression increased after 12 h of treatment with these metals, suggesting its involvement in the plant response to heavy metal stress. LmSAP transgenic tobacco plants subjected to these stress conditions were healthy, experienced higher seedling survival rates, and had longer roots than non-transgenic plants (NT). However, they exhibited higher tolerance towards cadmium and manganese than towards copper and zinc. LmSAP-overexpressing tobacco seedlings accumulated more cadmium, copper, and manganese compared with NT plants, but displayed markedly decreased hydrogen peroxide (H2O2) and lipid peroxidation levels after metal treatment. Activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were significantly higher in transgenic plants than in NT plants after exposure to metal stress. LmSAP overexpression also enhanced the transcription of several genes encoding metallothioneins (Met1, Met2, Met3, Met4, and Met5), a copper transport protein CCH, a Cys and His-rich domain-containing protein RAR1 (Rar1), and a ubiquitin-like protein 5 (PUB1), which are involved in metal tolerance in tobacco. Our findings indicate that LmSAP overexpression in tobacco enhanced tolerance to heavy metal stress by protecting the plant cells against oxidative stress, scavenging reactive oxygen species (ROS), and decreasing the intracellular concentration of free heavy metals through its effect on metal-binding proteins in the cytosol
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|a Journal Article
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|a Heavy metal tolerance
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|a LmSAP gene
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|a Lobularia maritima
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|a Metal accumulation
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|a Oxidative stress
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| 650 |
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|a Metals, Heavy
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Reactive Oxygen Species
|2 NLM
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| 650 |
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|a Soil Pollutants
|2 NLM
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| 700 |
1 |
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|a Hsouna, Anis Ben
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Saibi, Walid
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hamed, Karim Ben
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Brini, Faical
|e verfasserin
|4 aut
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|a Ghneim-Herrera, Thaura
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 231(2018) vom: 11. Dez., Seite 234-243
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:231
|g year:2018
|g day:11
|g month:12
|g pages:234-243
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|u http://dx.doi.org/10.1016/j.jplph.2018.09.019
|3 Volltext
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|d 231
|j 2018
|b 11
|c 12
|h 234-243
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