T-blocker : a simple and robust probe-free quantitative PCR assay to detect somatic mutations down to 0.1% frequency
We have developed a simple and robust probe-free quantitative PCR (qPCR) assay method that can detect minor mutant alleles with a frequency as low as 0.1% in a heterogeneous sample by introducing a novel T-blocker concept to the allele-specific PCR method. Four new KRAS and BRAF mutation detection a...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 65(2018), 4 vom: 02. Okt., Seite 205-210
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| 1. Verfasser: |
Kim, Hanyoup
(VerfasserIn) |
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| Weitere Verfasser: |
Ruby, Aaron E,
Shandilya, Harini G,
Virmani, Arvind K,
Rahman, Nandita,
Strange, Christina M,
Huuskonen, Jarkko |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
T-blocker
intercalating dye
qPCR
somatic mutation detection
Coloring Agents
Intercalating Agents
KRAS protein, human
DNA
9007-49-2
mehr...
BRAF protein, human
EC 2.7.11.1
Proto-Oncogene Proteins B-raf
Proto-Oncogene Proteins p21(ras)
EC 3.6.5.2 |
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