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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b01272
|2 doi
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|a pubmed24n0961.xml
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|a (DE-627)NLM288492080
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|a (NLM)30212214
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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1 |
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|a Nolles, Antsje
|e verfasserin
|4 aut
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|a FRET Reveals the Formation and Exchange Dynamics of Protein-Containing Complex Coacervate Core Micelles
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 04.01.2019
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|a Date Revised 28.12.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The encapsulation of proteins into complex coacervate core micelles (C3Ms) is of potential interest for a wide range of applications. To address the stability and dynamic properties of these polyelectrolyte complexes, combinations of cyan, yellow, and blue fluorescent proteins were encapsulated with cationic-neutral diblock copolymer poly(2-methyl-vinyl-pyridinium)128- b-poly(ethylene-oxide)477. Förster resonance energy transfer (FRET) allowed us to determine the kinetics of C3M formation and of protein exchange between C3Ms. Both processes follow first-order kinetics with relaxation times of ±100 s at low ionic strength ( I = 2.5 mM). Stability studies revealed that 50% of FRET was lost at I = 20 mM, pointing to the disintegration of the C3Ms. On the basis of experimental and theoretical considerations, we propose that C3Ms relax to their final state by association and dissociation of near-neutral soluble protein-polymer complexes. To obtain protein-containing C3Ms suitable for applications, it is necessary to improve the rigidity and salt stability of these complexes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cyan Fluorescent Protein
|2 NLM
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|a Micelles
|2 NLM
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|a Polyvinyls
|2 NLM
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|a Green Fluorescent Proteins
|2 NLM
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|a 147336-22-9
|2 NLM
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|a Polyethylene Glycols
|2 NLM
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|a 3WJQ0SDW1A
|2 NLM
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|a Sodium Chloride
|2 NLM
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|a 451W47IQ8X
|2 NLM
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| 700 |
1 |
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|a Hooiveld, Ellard
|e verfasserin
|4 aut
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|a Westphal, Adrie H
|e verfasserin
|4 aut
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|a van Berkel, Willem J H
|e verfasserin
|4 aut
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|a Kleijn, J Mieke
|e verfasserin
|4 aut
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|a Borst, Jan Willem
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 34(2018), 40 vom: 09. Okt., Seite 12083-12092
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:34
|g year:2018
|g number:40
|g day:09
|g month:10
|g pages:12083-12092
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|u http://dx.doi.org/10.1021/acs.langmuir.8b01272
|3 Volltext
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