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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b01298
|2 doi
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|a pubmed24n0961.xml
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|a (DE-627)NLM288458737
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|a (NLM)30208711
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Saini, Gaurav
|e verfasserin
|4 aut
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|a Performance Comparison of Three Chemical Vapor Deposited Aminosilanes in Peptide Synthesis
|b Effects of Silane on Peptide Stability and Purity
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 17.12.2018
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|a Date Revised 17.12.2018
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Silicon oxide substrates underwent gas-phase functionalization with various aminosilanes, and the resulting surfaces were evaluated for their suitability as a solid support for solid phase peptide synthesis (SPPS). APTES (3-aminopropyltriethoxysilane), APDEMS (3-aminopropyldiethoxymethylsilane), and APDIPES (3-aminopropyldiisopropylethoxysilane) were individually applied to thermal oxide-terminated silicon substrates via gas-phase deposition. Coated surfaces were characterized by spectroscopic ellipsometry (SE), contact angle goniometry, X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and spectrophotometry. Model oligopeptides with 16 residues were synthesized on the amino surfaces, and the chemical stabilities of the resulting surfaces were evaluated against a stringent side chain deprotection (SCD) step, which contained trifluoroacetic acid (TFA) and trifluoromethanesulfonic acid (TFMSA). Functionalized surface thickness loss during SCD was most acute for APDIPES and the observed relative stability order was APTES > APDEMS > APDIPES. Amino surfaces were evaluated for compatibility with stepwise peptide synthesis where complete deprotection and coupling cycles are paramount. Model trimer syntheses indicated that routine capping of unreacted amines with acetic anhydride significantly increased purity as measured by MALDI-MS. An inverse correlation between the amine loading density and peptide purity was observed. In general, peptide purity was highest for the lowest amine density APDIPES surface
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|a Journal Article
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|a Trenchevska, Olgica
|e verfasserin
|4 aut
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1 |
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|a Howell, Loren J
|e verfasserin
|4 aut
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|a Boyd, James G
|e verfasserin
|4 aut
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| 700 |
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|a Smith, David P
|e verfasserin
|4 aut
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|a Jain, Varun
|e verfasserin
|4 aut
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|a Linford, Matthew R
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 34(2018), 40 vom: 09. Okt., Seite 11925-11932
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:34
|g year:2018
|g number:40
|g day:09
|g month:10
|g pages:11925-11932
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|u http://dx.doi.org/10.1021/acs.langmuir.8b01298
|3 Volltext
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