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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-03-18-0496-RE
|2 doi
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|a pubmed24n0961.xml
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|a (DE-627)NLM288447042
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|a (NLM)30207510
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Cieniewicz, Elizabeth
|e verfasserin
|4 aut
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|a Prevalence and Genetic Diversity of Grabloviruses in Free-Living Vitis spp
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 21.02.2019
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|a Date Revised 21.02.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The distribution and diversity of grapevine red blotch virus (GRBV) and wild Vitis virus 1 (WVV1) (genus Grablovirus; family Geminiviridae) were determined in free-living Vitis spp. in northern California and New York from 2013 to 2017. Grabloviruses were detected by polymerase chain reaction in 28% (57 of 203) of samples from California but in none of the 163 samples from New York. The incidence of GRBV in free-living vines was significantly higher in samples from California counties with high compared with low grape production (χ2 = 83.09; P < 0.001), and in samples near (<5 km) to compared with far (>5 km) from vineyards (χ2 = 57.58; P < 0.001). These results suggested a directional spread of GRBV inoculum predominantly from vineyards to free-living Vitis spp. WVV1 incidence was also significantly higher in areas with higher grape production acreage (χ2 = 16.02; P < 0.001). However, in contrast to GRBV, no differential distribution of WVV1 incidence was observed with regard to distance from vineyards (χ2 = 0.88; P = 0.3513). Two distinct phylogenetic clades were identified for both GRBV and WVV1 isolates from free-living Vitis spp., although the nucleotide sequence variability of the genomic diversity fragment was higher for WWV1 (94.3 to 99.8% sequence identity within clade 1 isolates and 90.1 to 100% within clade 2 isolates) than GRBV (98.3% between clade 1 isolates and 96.9 to 100% within clade 2 isolates). Additionally, evidence for intraspecific recombination events was found in WVV1 isolates and confirmed in GRBV isolates. The prevalence of grabloviruses in California free-living vines highlights the need for vigilance regarding potential grablovirus inoculum sources in order to protect new vineyard plantings and foundation stock vineyards in California
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Thompson, Jeremy R
|e verfasserin
|4 aut
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|a McLane, Heather
|e verfasserin
|4 aut
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|a Perry, Keith L
|e verfasserin
|4 aut
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|a Dangl, Gerald S
|e verfasserin
|4 aut
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|a Corbett, Quinlan
|e verfasserin
|4 aut
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|a Martinson, Timothy
|e verfasserin
|4 aut
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|a Wise, Alice
|e verfasserin
|4 aut
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|a Wallis, Anna
|e verfasserin
|4 aut
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|a O'Connell, James
|e verfasserin
|4 aut
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|a Dunst, Rick
|e verfasserin
|4 aut
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|a Cox, Kerik
|e verfasserin
|4 aut
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|a Fuchs, Marc
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant disease
|d 1997
|g 102(2018), 11 vom: 12. Nov., Seite 2308-2316
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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|g volume:102
|g year:2018
|g number:11
|g day:12
|g month:11
|g pages:2308-2316
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|u http://dx.doi.org/10.1094/PDIS-03-18-0496-RE
|3 Volltext
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a AR
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|d 102
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|e 11
|b 12
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|h 2308-2316
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