Prevalence and Genetic Diversity of Grabloviruses in Free-Living Vitis spp

The distribution and diversity of grapevine red blotch virus (GRBV) and wild Vitis virus 1 (WVV1) (genus Grablovirus; family Geminiviridae) were determined in free-living Vitis spp. in northern California and New York from 2013 to 2017. Grabloviruses were detected by polymerase chain reaction in 28%...

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Veröffentlicht in:Plant disease. - 1997. - 102(2018), 11 vom: 12. Nov., Seite 2308-2316
1. Verfasser: Cieniewicz, Elizabeth (VerfasserIn)
Weitere Verfasser: Thompson, Jeremy R, McLane, Heather, Perry, Keith L, Dangl, Gerald S, Corbett, Quinlan, Martinson, Timothy, Wise, Alice, Wallis, Anna, O'Connell, James, Dunst, Rick, Cox, Kerik, Fuchs, Marc
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.
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520 |a The distribution and diversity of grapevine red blotch virus (GRBV) and wild Vitis virus 1 (WVV1) (genus Grablovirus; family Geminiviridae) were determined in free-living Vitis spp. in northern California and New York from 2013 to 2017. Grabloviruses were detected by polymerase chain reaction in 28% (57 of 203) of samples from California but in none of the 163 samples from New York. The incidence of GRBV in free-living vines was significantly higher in samples from California counties with high compared with low grape production (χ2 = 83.09; P < 0.001), and in samples near (<5 km) to compared with far (>5 km) from vineyards (χ2 = 57.58; P < 0.001). These results suggested a directional spread of GRBV inoculum predominantly from vineyards to free-living Vitis spp. WVV1 incidence was also significantly higher in areas with higher grape production acreage (χ2 = 16.02; P < 0.001). However, in contrast to GRBV, no differential distribution of WVV1 incidence was observed with regard to distance from vineyards (χ2 = 0.88; P = 0.3513). Two distinct phylogenetic clades were identified for both GRBV and WVV1 isolates from free-living Vitis spp., although the nucleotide sequence variability of the genomic diversity fragment was higher for WWV1 (94.3 to 99.8% sequence identity within clade 1 isolates and 90.1 to 100% within clade 2 isolates) than GRBV (98.3% between clade 1 isolates and 96.9 to 100% within clade 2 isolates). Additionally, evidence for intraspecific recombination events was found in WVV1 isolates and confirmed in GRBV isolates. The prevalence of grabloviruses in California free-living vines highlights the need for vigilance regarding potential grablovirus inoculum sources in order to protect new vineyard plantings and foundation stock vineyards in California 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
700 1 |a Thompson, Jeremy R  |e verfasserin  |4 aut 
700 1 |a McLane, Heather  |e verfasserin  |4 aut 
700 1 |a Perry, Keith L  |e verfasserin  |4 aut 
700 1 |a Dangl, Gerald S  |e verfasserin  |4 aut 
700 1 |a Corbett, Quinlan  |e verfasserin  |4 aut 
700 1 |a Martinson, Timothy  |e verfasserin  |4 aut 
700 1 |a Wise, Alice  |e verfasserin  |4 aut 
700 1 |a Wallis, Anna  |e verfasserin  |4 aut 
700 1 |a O'Connell, James  |e verfasserin  |4 aut 
700 1 |a Dunst, Rick  |e verfasserin  |4 aut 
700 1 |a Cox, Kerik  |e verfasserin  |4 aut 
700 1 |a Fuchs, Marc  |e verfasserin  |4 aut 
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