Transient genetic transformation of Mougeotia scalaris (Zygnematophyceae) mediated by the endogenous α-tubulin1 promoter
© 2018 Phycological Society of America.
| Publié dans: | Journal of phycology. - 1966. - 54(2018), 6 vom: 01. Dez., Seite 840-849 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2018
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| Accès à la collection: | Journal of phycology |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Mougeotia scalaris biolistic particle delivery cell division genetic transformation green fluorescent protein streptophyte algae tubulin Algal Proteins |
| Résumé: | © 2018 Phycological Society of America. Mougeotia scalaris is a filamentous streptophyte alga renowned for light-inducible plastid rotation and microtubule-dependent polarity establishment. As a first step toward transgenic approaches we determined the 5,825 base pair genomic sequence encoding the α-tubulin1 gene (MsTUA1) of M. scalaris (strain SAG 164.80). The subcloned MsTUA1 promoter facilitated strong transgene expression in M. scalaris and tobacco leaf cells, as shown by particle bombardment and the subsequent visualization of expressed fluorescent protein markers. Our results provide a route for the genetic transformation of the filamentous streptophyte alga M. scalaris based on the endogenous TUA1 promoter |
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| Description: | Date Completed 17.09.2019 Date Revised 09.04.2022 published: Print-Electronic GENBANK: AF441490.1, MG680733, MG680734 Citation Status MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/jpy.12781 |