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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b01635
|2 doi
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|a pubmed25n0960.xml
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|a (NLM)30165742
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Dittrich, Matthias
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Interactions of Cationic Lipids with DNA
|b A Structural Approach
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 05.08.2019
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|a Date Revised 05.08.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Colloidal nucleic acid carrier systems based on cationic lipids are a promising pharmaceutical tool in the implementation of gene therapeutic strategies. This study demonstrates the complex behavior of DNA at the lipid-solvent interface facilitating structural changes of the lyotropic liquid-crystalline phases. For this study, the structural properties of six malonic acid based cationic lipids were determined using small- and wide-angle X-ray scattering (SAXS and WAXS) as well as differential scanning calorimetry (DSC). Selected lipids (lipid 3 and lipid 6) with high nucleic acid transfer activity have been investigated in detail because of the strong influence of the zwitterionic helper lipid 1,2-di(9 Z-octadecenoyl)- sn-glycero-3-phosphoethanolamine (DOPE) on the structural properties as well as of the complex formation of lipid-DNA complexes (lipoplexes). In the case of lipid 3, DNA stabilizes a metastable cubic mesophase with Im3 m symmetry and an Im3 m Qαc lipoplex is formed, which is rarely described for DNA lipoplexes in literature. In the case of lipid 6, a cubic mesophase with Im3 m symmetry turns into a fluid lamellar phase while mixing with DOPE and complexing DNA
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Amides
|2 NLM
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|a Fatty Acids, Unsaturated
|2 NLM
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|a Malonates
|2 NLM
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Brauer, Chris
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Funari, Sergio S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dobner, Bodo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Brezesinski, Gerald
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wölk, Christian
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 34(2018), 49 vom: 11. Dez., Seite 14858-14868
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:34
|g year:2018
|g number:49
|g day:11
|g month:12
|g pages:14858-14868
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|u http://dx.doi.org/10.1021/acs.langmuir.8b01635
|3 Volltext
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