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231225s2018 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2018-0017
|2 doi
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|a eng
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1 |
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|a Park, Ah-Mee
|e verfasserin
|4 aut
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|a Forensic luminol reaction for detecting fecal occult blood in experimental mice
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 05.08.2019
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|a Date Revised 09.03.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Fecal occult blood (FOB) is a sign of gastrointestinal diseases, such as intestinal ulcers and colorectal cancer. In experimental animal studies, there is no standard method to detect FOB. Here, we present a simple protocol to detect FOB in mice, using the Luminol Reaction Experiment Kit® that was originally designed to detect bloodstains at a crime scene in criminal forensics. To obtain positive control bloody feces, we used an indomethacin-induced intestinal ulcer model in mice. By mixing small pieces of feces with a luminol solution, the fecal solution emitted visible blue-white chemiluminescence in dark field when feces contained hemoglobin. We also established a method for semi-quantification of hemoglobin content in the fecal solution, using a luminometer. This method is simple, quick, economical and semi-quantitative, allowing researchers to detect FOB in experimental mice
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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4 |
|a animals
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|a gastrointestinal hemorrhage
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|a laboratory
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|a luminescence
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|a stool
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|a Luminescent Agents
|2 NLM
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|a Luminol
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| 650 |
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|a 5EXP385Q4F
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1 |
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|a Tsunoda, Ikuo
|e verfasserin
|4 aut
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|i Enthalten in
|t BioTechniques
|d 1993
|g 65(2018), 4 vom: 10. Okt., Seite 227-230
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
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|g volume:65
|g year:2018
|g number:4
|g day:10
|g month:10
|g pages:227-230
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| 856 |
4 |
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|u http://dx.doi.org/10.2144/btn-2018-0017
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