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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ery278
|2 doi
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|a pubmed24n0958.xml
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Chu, Mingxue
|e verfasserin
|4 aut
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|a AtCaM4 interacts with a Sec14-like protein, PATL1, to regulate freezing tolerance in Arabidopsis in a CBF-independent manner
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.10.2019
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|a Date Revised 15.10.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a Calmodulin (CaM), a multifunctional Ca2+ sensor, mediates multiple reactions involved in regulation of plant growth and responses to environmental stress. In this study, we found that AtCaM4 plays a negative role in freezing tolerance in Arabidopsis. The deletion of AtCaM4 resulted in enhanced freezing tolerance in cam4 mutant plants. Although AtCaM4 and AtCaM1 were cold-induced isoforms, cam4/cam1Ri double-mutant and cam4 single-mutant plants exhibited similar improvements in freezing tolerance, indicating that AtCaM4 plays major role. Furthermore, we found that AtCaM4 may influence freezing tolerance in a C-repeat binding factor (CBF)-independent manner as cold-induced expression patterns of CBFs did not change in the cam4/cam1Ri mutant. In addition, among the cold-responsive (COR) genes detected, KIN1, COR15b, and COR8.6 exhibited clearly enhanced expression over the long term in cam4/cam1Ri mutant plants exposed to cold stress. Using immunoprecipitation and mass spectrometry, we identified multiple candidate AtCaM4-interacting proteins. Co-immunoprecipitation assays confirmed the interaction of AtCaM4 with PATL1 in vivo and a phenotype analysis showed that patl1 mutant plants exhibited enhanced freezing tolerance. Thus, we conclude that AtCaM4 negatively regulates freezing tolerance in Arabidopsis by interacting with the novel CaM-binding protein PATL1
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis Proteins
|2 NLM
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|a CAM4 protein, Arabidopsis
|2 NLM
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| 650 |
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|a Calmodulin
|2 NLM
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| 650 |
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|a PATL1 protein, Arabidopsis
|2 NLM
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| 650 |
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|a Phospholipid Transfer Proteins
|2 NLM
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| 700 |
1 |
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|a Li, Jiaojiao
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Jingyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shen, Sufen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Cuina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gao, Yingjie
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Suqiao
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 69(2018), 21 vom: 12. Okt., Seite 5241-5253
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:69
|g year:2018
|g number:21
|g day:12
|g month:10
|g pages:5241-5253
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|u http://dx.doi.org/10.1093/jxb/ery278
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