Transcriptomic comparison of two barley genotypes differing in arsenic tolerance exposed to arsenate and phosphate treatments
Copyright © 2018 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 130(2018) vom: 30. Sept., Seite 589-603 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2018
|
| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Comparative Study Journal Article Arsenate Differentially expressed genes Down regulation Phosphate Transcriptomics Up regulation Arsenates Phosphate Transport Proteins mehr... |
| Zusammenfassung: | Copyright © 2018 Elsevier Masson SAS. All rights reserved. Arsenic (As) is a ubiquitous metalloid and toxic to plants. Chemical similarity between arsenate and phosphate (P) indicates possible antagonism between them in uptake and transportation. However, there is little study to reveal the interaction of As and P at transcriptional level. In this study RNA-sequencing was conducted on the two barley genotypes differing in As tolerance. A total of 2942 differentially expressed genes (DEGs) were inclusively expressed in both genotypes under As (100 μM) and As (100 μM) + P (50 μM), and these DEGs included hormonal signaling, stress responsive, transport related and transcription factors. P addition in the culture solution inhibited the KEGG pathways related to ABC transporters, ether lipid metabolism, linolenic acid metabolism, endocytosis and RNA transport. ZDB160 had a higher expression of DEGs associated with hormone signaling, secondary metabolites and stress defense under P conditions compared to ZDB475, which might explain its tolerance mechanism to As under P condition. The abscisic acid, jasmonic acid and salicylic acid signaling pathways were also significantly regulated under As + P conditions, which may also account for genotypic differences. Finally we drew up a hypothetical model of high As + P stress tolerance mechanism in ZDB160. It may be concluded that ZDB160 achieves its tolerance to As under P by up-regulating P transporters, resulting in more P uptake and less As translocation. The identified candidate genes related to As + P tolerance may provide insights into understanding As tolerance under limited P conditions |
|---|---|
| Beschreibung: | Date Completed 22.10.2018 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2018.08.006 |