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231225s2018 xx |||||o 00| ||eng c |
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7 |
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|a 10.1270/jsbbs.17103
|2 doi
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|a eng
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1 |
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|a Chiou, Wan-Yi
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Easy sectioning of whole grain of rice using cryomicrotome
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| 264 |
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1 |
|c 2018
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Revised 01.10.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a To obtain a clear intact section of a ripened rice grain, which is suitable for biochemical and histological analysis, the Kawamoto method using a specific adhesive film was applied using a cryomicrotome. The longitudinal and sagittal sections were easily obtained together with the cross-section, and cell characteristics were clearly discerned in the ripened grain. It was demonstrated that the Kawamoto method is readily applicable for intact sectioning of hard tissue, including ripened grain. Intact section sampling may be useful for enzymatic analysis and transcriptomic analysis of plant tissue
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|a Journal Article
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|a cross section
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|a cryomicrotome
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|a easy sectioning
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| 650 |
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|a longitudinal section
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4 |
|a matured grain
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| 650 |
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|a rice
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| 700 |
1 |
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|a Tsugane, Kazuo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kawamoto, Tadafumi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Maekawa, Masahiko
|e verfasserin
|4 aut
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| 773 |
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8 |
|i Enthalten in
|t Breeding science
|d 1998
|g 68(2018), 3 vom: 10. Juni, Seite 381-384
|w (DE-627)NLM098238299
|x 1344-7610
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|g volume:68
|g year:2018
|g number:3
|g day:10
|g month:06
|g pages:381-384
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|u http://dx.doi.org/10.1270/jsbbs.17103
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