Visualization of global RNA synthesis in a human (mini-) organ in situ by click chemistry
RNA synthesis can be detected by 5-ethynyl uridine (EU) incorporation and click chemistry. Despite identifying a fundamental functional process, this technique has yet to be widely applied to complex human tissue systems. By incorporating EU into human hair follicle (HF) organs cultured ex vivo, nas...
Ausführliche Beschreibung
Bibliographische Detailangaben
Veröffentlicht in: | BioTechniques. - 1993. - 65(2018), 2 vom: 01. Aug., Seite 97-100
|
1. Verfasser: |
Purba, Talveen S
(VerfasserIn) |
Weitere Verfasser: |
Marsh, Jack,
Ng'andu, Kayumba,
Kurinna, Svitlana,
Paus, Ralf |
Format: | Online-Aufsatz
|
Sprache: | English |
Veröffentlicht: |
2018
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Zugriff auf das übergeordnete Werk: | BioTechniques
|
Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
5-ethynyl uridine
O-propargyl-puromycin
RNA synthesis
click chemistry
human hair follicle
protein synthesis
RNA
63231-63-0
mehr...
Uridine
WHI7HQ7H85 |