Calcium Ions Affect Water Molecular Structures Surrounding an Oligonucleotide Duplex as Revealed by Sum Frequency Generation Vibrational Spectroscopy
The solvation of DNA in water facilitates the formation of a hydration layer surrounding it, thus stabilizing the DNA duplex in the biological aqueous environment. In this study, via using the lipid bilayer as a soft substrate to accommodate the duplex oligonucleotide, the structure of the water lay...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 34(2018), 49 vom: 11. Dez., Seite 14774-14779 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Lipid Bilayers Oligodeoxyribonucleotides Water 059QF0KO0R 1,2-Dipalmitoylphosphatidylcholine 2644-64-6 Cholesterol 97C5T2UQ7J mehr... |
| Zusammenfassung: | The solvation of DNA in water facilitates the formation of a hydration layer surrounding it, thus stabilizing the DNA duplex in the biological aqueous environment. In this study, via using the lipid bilayer as a soft substrate to accommodate the duplex oligonucleotide, the structure of the water layer surrounding the oligonucleotide was detected under the perturbation of the calcium ions (Ca2+) with chiral and achiral sum frequency generation (SFG) vibrational spectroscopy. With increasing Ca2+ concentration, both the chiral and achiral water vibrational signals had similar concentration-dependent changes, i.e., an initial decreasing phase followed by an increasing phase. However, when the Ca2+ concentrations were adjusted to within the range comparable to those in the human serum, the chiral water vibrational signals remained nearly unchanged, whereas the achiral water vibrational signals still changed as a function of the Ca2+ concentration. Therefore, the current experimental result supports the possible protection function of the chiral hydration layer against the Ca2+ ions, which generally exist in the cell sap and play important roles in many biological functions |
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| Beschreibung: | Date Completed 05.08.2019 Date Revised 05.08.2019 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/acs.langmuir.8b01763 |