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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b01347
|2 doi
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|a pubmed25n0957.xml
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|a eng
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| 100 |
1 |
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|a Carvalho, Ana Margarida
|e verfasserin
|4 aut
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| 245 |
1 |
3 |
|a An Anticaffeine Antibody-Oligonucleotide Conjugate for DNA-Directed Immobilization in Environmental Immunoarrays
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| 264 |
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 05.08.2019
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|a Date Revised 05.08.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The development of fast and cheap high-throughput platforms for the detection of environmental contaminants is of particular importance to understand the human-related impact on the environment. The application of DNA-directed immobilization (DDI) of IgG molecules is currently limited to the clinical diagnostics scenario, possibly because of the high costs of production of such addressable platforms. We here describe the efficient and specific hybridization of an antibody-oligonucleotide conjugate to a short 12-mer capture probe. The specific antibody used is a monoclonal antibody against caffeine, a stimulant and important anthropogenic marker. With this work, we hope to contribute to broadening the application potential of DDI to environmental markers in order to develop cheaper and more stable high-throughput screening platforms for standard routine analysis of pollutants in a variety of complex matrices
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Antibodies, Monoclonal
|2 NLM
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| 650 |
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7 |
|a Central Nervous System Stimulants
|2 NLM
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| 650 |
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7 |
|a DNA Probes
|2 NLM
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| 650 |
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7 |
|a Immobilized Nucleic Acids
|2 NLM
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| 650 |
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7 |
|a Oligodeoxyribonucleotides
|2 NLM
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| 650 |
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7 |
|a Water Pollutants, Chemical
|2 NLM
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| 650 |
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7 |
|a Caffeine
|2 NLM
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| 650 |
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7 |
|a 3G6A5W338E
|2 NLM
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| 650 |
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|a DNA
|2 NLM
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| 650 |
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7 |
|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Montes, Cinthya Véliz
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schneider, Rudolf J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Madder, Annemieke
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 34(2018), 49 vom: 11. Dez., Seite 14834-14841
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:34
|g year:2018
|g number:49
|g day:11
|g month:12
|g pages:14834-14841
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|u http://dx.doi.org/10.1021/acs.langmuir.8b01347
|3 Volltext
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