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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b01597
|2 doi
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|a pubmed24n0957.xml
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|a (DE-627)NLM287259013
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|a (NLM)30086642
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Fontes, Cassio M
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Engineering the Surface Properties of a Zwitterionic Polymer Brush to Enable the Simple Fabrication of Inkjet-Printed Point-of-Care Immunoassays
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.01.2020
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|a Date Revised 05.02.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Motivated by the lack of adventitious protein adsorption on zwitterionic polymer brushes that promise low noise and hence high analytical sensitivity for surface-based immunoassays, we explored their use as a substrate for immunoassay fabrication by the inkjet printing of antibodies. We observed that a poly(sulfobetaine)methacrylate brush on glass is far too hydrophilic to enable the noncovalent immobilization of antibodies by inkjet printing. To circumvent this limitation, we developed a series of hybrid zwitterionic-cationic surface coatings with tunable surface wettability that are suitable for the inkjet printing of antibodies but also have low protein adsorption. We show that in a microarray format in which both the capture and detection antibodies are discretely printed as spots on these hybrid brushes, a point-of-care sandwich immunoassay can be carried out with an analytical sensitivity and dynamic range that is similar to or better than those of the same assay fabricated on a PEG-like brush. We also show that the hybrid polymer brushes do not bind anti-PEG antibodies that are ubiquitous in human blood, which can be a problem with immunoassays fabricated on PEG-like coatings
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Antibodies, Immobilized
|2 NLM
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|a IL6 protein, human
|2 NLM
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|a Interleukin-6
|2 NLM
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|a Methacrylates
|2 NLM
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|a sulfobetaine methacrylate polymer
|2 NLM
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|a Achar, Rohan K
|e verfasserin
|4 aut
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|a Joh, Daniel Y
|e verfasserin
|4 aut
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| 700 |
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|a Ozer, Imran
|e verfasserin
|4 aut
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|a Bhattacharjee, Somnath
|e verfasserin
|4 aut
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| 700 |
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|a Hucknall, Angus
|e verfasserin
|4 aut
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| 700 |
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|a Chilkoti, Ashutosh
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 35(2019), 5 vom: 05. Feb., Seite 1379-1390
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:35
|g year:2019
|g number:5
|g day:05
|g month:02
|g pages:1379-1390
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|u http://dx.doi.org/10.1021/acs.langmuir.8b01597
|3 Volltext
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