Antigenotoxic potential of plant leaf extracts of Parkinsonia aculeata L. using Allium cepa assay
Copyright © 2018 Elsevier Masson SAS. All rights reserved.
| Publié dans: | Plant physiology and biochemistry : PPB. - 1991. - 130(2018) vom: 01. Sept., Seite 314-323 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2018
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| Accès à la collection: | Plant physiology and biochemistry : PPB |
| Sujets: | Journal Article Allium cepa Antigenotoxic Maleic hydrazide P. aculeata Plant Extracts Maleic Hydrazide TQ7L3FCV66 |
| Résumé: | Copyright © 2018 Elsevier Masson SAS. All rights reserved. The aim of the present study was to evaluate the antigenotoxic potential of P. aculeata L. leaf extract/fractions against maleic hydrazide (MH) using Allium cepa root chromosomal aberration assay. The excessive reduction in root growth and mitotic index value was observed after 3 h treatment of MH as compared to negative control (water). In case of MH treatment, frequency of aberrated cells significantly (p ≤ 0.05) raised from 129 to 337 at 0.1 ppm and 2.0 ppm concentrations respectively. From root growth inhibition test with MH treatment, EC50 value i.e. 0.5 ppm was selected to study the antigenotoxic effect of different extract/fractions of P. aculeata L. leaves. All the extract/fractions showed increase in mitotic index and great reduction in chromosomal aberrations with rise in concentration against the genotoxicity of MH. Among all the extract/fractions, butanol and ethyl acetate fractions showed significant reduction in chromosomal aberrations in A. cepa cells and indicates the chemo preventive activity. Antigenotoxic property of this plant is due to the presence of various phytochemicals in leaf such as epi-orientin, Parkinsonin-A, Parkinsonin-B, orientin, iso-orientin, vitexin, iso-vitexin, C-glycosylflavone, parkintin, rotenoids, terpenoids, flavonoids, saponins, alkaloids, glycosides and anthraquinone etc. Our result showed that among all the treatments, simultaneous treatment showed best result followed by pre and post treatment. Further studies in animal model are suggested for further evaluation of the use of P. aculeata leaf extract in human welfare |
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| Description: | Date Completed 22.10.2018 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2018.07.017 |