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231225s2018 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2018-0014
|2 doi
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|a pubmed25n0955.xml
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|a eng
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| 100 |
1 |
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|a Ryu, Jun Hyung
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Non-lethal method for the preparation of metaphase spreads using cultured mantle tissue from live adult abalone
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 08.08.2019
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|a Date Revised 08.08.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a Metaphase spread preparation in adult abalone has not been successful, which has restricted the applications of karyotyping-based technologies. Here, we present a non-lethal method to enable preparation of metaphase spreads from live adult abalone using a tissue culture method. Mantle tissue fragments from live adult abalone were cultured in vitro and the cultured cells were used for metaphase spread preparation. To retrieve a sufficient number of proliferating cells required for metaphase spread preparation, at least 14 days of culture was required, and culturing the marginal zone of mantle was more optimal than culturing other areas. Additionally, it was shown that simple medium consisting of basal medium, fetal bovine serum and antibiotics could stimulate cellular proliferation followed by metaphase spread preparation
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4 |
|a Journal Article
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|a Research Support, Non-U.S. Gov't
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4 |
|a adult abalone
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| 650 |
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4 |
|a culture
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| 650 |
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4 |
|a karyotyping
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| 650 |
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4 |
|a mantle
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| 650 |
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4 |
|a metaphase spreads
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| 700 |
1 |
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|a Lee, Sang Yoon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nam, Yoon Kwon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gong, Seung Pyo
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t BioTechniques
|d 1993
|g 65(2018), 1 vom: 01. Juli, Seite 33-36
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:65
|g year:2018
|g number:1
|g day:01
|g month:07
|g pages:33-36
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|u http://dx.doi.org/10.2144/btn-2018-0014
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