Effect of quorum quenching bacteria on growth, virulence factors and biofilm formation of Yersinia ruckeri in vitro and an in vivo evaluation of their probiotic effect in rainbow trout

© 2018 John Wiley & Sons Ltd.

Bibliographische Detailangaben
Veröffentlicht in:Journal of fish diseases. - 1998. - 41(2018), 9 vom: 30. Sept., Seite 1429-1438
1. Verfasser: Torabi Delshad, Somayeh (VerfasserIn)
Weitere Verfasser: Soltanian, Siyavash, Sharifiyazdi, Hassan, Bossier, Peter
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Journal of fish diseases
Schlagworte:Journal Article Yersinia ruckeri biofilm enteric redmouth disease probiotic bacteria Culture Media Virulence Factors homoserine lactone 1192-20-7 4-Butyrolactone OL659KIY4X
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245 1 0 |a Effect of quorum quenching bacteria on growth, virulence factors and biofilm formation of Yersinia ruckeri in vitro and an in vivo evaluation of their probiotic effect in rainbow trout 
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520 |a Five N-acyl homoserine lactone-degrading bacteria (quorum quenching (QQ) strains) were selected to evaluate their impacts on growth, virulence factors and biofilm formation in Yersinia ruckeri in vitro. No difference was observed among the growth pattern of Y. ruckeri in monoculture and coculture with the QQ strains. To investigate the regulation of virulence factors by quorum sensing in Y. ruckeri, cultures were supplemented with 3oxo-C8-HSL. The results indicated that swimming motility and biofilm formation are positively regulated by QS (p < 0.05), whereas caseinase, phospholipase and haemolysin productions are not influenced by 3oxo-C8-HSL (p > 0.05). The QQs were able to decrease swimming motility and biofilm formation in Y. ruckeri. QQ bacteria were supplemented to trout feed at 108  CFU/g (for 40 days). Their probiotic effect was verified by Y. ruckeri challenge either by immersion or injection in trout. All strains could significantly increase fish survival with Bacillus thuringiensis and Citrobacter gillenii showing the highest and lowest relative percentage survival (RPS) values (respectively, 85% and 38%). Besides, there was no difference between the RPS values by either immersion or injection challenge expect for B. thuringiensis. The putative involvement of the QQ capacity in the protection against Yersinia is discussed 
650 4 |a Journal Article 
650 4 |a Yersinia ruckeri 
650 4 |a biofilm 
650 4 |a enteric redmouth disease 
650 4 |a probiotic bacteria 
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650 7 |a Virulence Factors  |2 NLM 
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700 1 |a Soltanian, Siyavash  |e verfasserin  |4 aut 
700 1 |a Sharifiyazdi, Hassan  |e verfasserin  |4 aut 
700 1 |a Bossier, Peter  |e verfasserin  |4 aut 
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