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231225s2018 xx |||||o 00| ||eng c |
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7 |
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|a 10.1111/jfd.12816
|2 doi
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|a pubmed25n0954.xml
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|a eng
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| 100 |
1 |
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|a Pao, H Y
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Development, characterization and virus susceptibility of a continuous cell line from the caudal fin of marbled eel (Anguilla marmorata)
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1 |
|c 2018
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 30.01.2019
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a GENBANK: MG762746, MG762748, MG762749, MG762750, MG762751
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|a Citation Status MEDLINE
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| 520 |
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|a © 2018 John Wiley & Sons Ltd.
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|a A continuous cell line consisting mostly of epithelioid cells was established from the caudal fin of marbled eels (Anguilla marmorata) and designated as marbled eel caudal fin (MECF)-1. The cells multiplied well in Leibovitz's L-15 medium containing 2% to 15% foetal bovine serum at temperatures of 20°C to 35°C and were subcultured for >90 passages during a 5-year period from 2012 to 2017. Transcripts of ictacalcin, keratin 13, cd146, nestin, ncam1 and myod1 were demonstrated in the cells using reverse transcription polymerase chain reaction. The results indicated that MECF-1 was composed of epidermal and mesenchyme stem and progenitor cells including myoblasts. MECF-1 was susceptible to Japanese eel herpesvirus HVA980811, marbled eel polyoma-like virus (MEPyV), aquabirnavirus MEIPNV1310 and aquareovirus CSV. By contrast, MECF-1 was noted refractory to megalocytiviruses RSIV-Ku and GSIV-K1 infection. Moreover, the cells were resistant to betanodavirus infection. In conclusion, MECF-1 derived from marbled eel is suitable for studies on anguillid viruses and interaction with host cells
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|a Journal Article
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|a Anguilla
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| 650 |
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|a IPNV
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| 650 |
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|a herpesvirus
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| 650 |
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4 |
|a marbled eel caudal fin-1
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| 650 |
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4 |
|a polyoma-like virus
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| 650 |
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|a Culture Media
|2 NLM
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| 700 |
1 |
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|a Wu, C Y
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huang, C H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wen, C M
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 41(2018), 9 vom: 30. Sept., Seite 1331-1338
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
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|g volume:41
|g year:2018
|g number:9
|g day:30
|g month:09
|g pages:1331-1338
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| 856 |
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|u http://dx.doi.org/10.1111/jfd.12816
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